Select AllDeselectExport
Display Method:
Reviews
Awareness and ability of paradigm shift are needed for research on dominant diseases of TCM
Yanjun Zhang
2023,15(4):475-475, DOI: https://doi.org/10.1016/j.chmed.2023.10.001
[Abstract] (39) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:

Clinical value-oriented research paradigm about inheritance and innovation development of TCM dominant diseases
Dongna Li a, b, 1, Hong Guo a, b, 1, Lin Niu a, b, Qingsheng Yin a, b, Yanjun Zhang c, d, *, Pengwei Zhuang a, b, *
2023,15(4):476-484, DOI: https://doi.org/10.1016/j.chmed.2023.09.002
[Abstract] (12) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Modern medicine has made remarkable achievements in safeguarding people’s life and health, however, it is increasingly found that in the face of complex diseases, selective targeting single target is often difficult to produce a comprehensive rehabilitation effect, and is prone to induce drug resistance, toxic side effects. Traditional Chinese medicine (TCM) has a long history of clinical application, and its clinical value in the treatment of complex diseases such as cardiovascular and cerebrovascular diseases, digestive diseases, skin diseases, rheumatism and immunity diseases, and adjuvant treatment of tumors has been proven to have obvious advantages. However, its modern research is relatively lagging behind, and in the face of the aging society and the characteristics of the modern disease spectrum, the traditional knowledge-driven research paradigm seems to be stuck in a bottleneck and difficult to make greater breakthroughs. Focusing on the key issues of TCM development in the new era, the clinical value-oriented strategy becomes to be a new research paradigm of TCM inheritance and innovation development, and dominant diseases would be the focus of the TCM inheritance and innovation development, which has been highly valued in recent years by the TCM academia and the relevant national management departments. Based on the clinical value, a series of policies are formulated for the selection and evaluation of the TCM dominant diseases (TCMDD), and exploratory researches about the clinical efficacy characteristics, the modern scientific connotation interpretation were carried out. The clinical value-oriented research paradigm of TCMDD inheritance and innovation development has been initially formed, which is characterized by strong policy support as the guarantee, systematic and standardized selection and evaluation methods as the driving force, scientific and effective research on internal mechanisms as the expansion, and effective clinical guidelines and principles as the transformation, which is of great value in promoting the high-quality development of the industries and undertaking of TCM. In this paper, the main policy support, selection and evaluation methods, therapeutic effect characterization, and modern scientific connotation research strategies of TCMDD in recent years have been comprehensively sorted out, with a view to providing the healthy and benign development of the research on TCMDD.
Chinese herbal medicines for prostate cancer therapy: From experimental research to clinical practice
Fanming Kong a, b, 1, Chaoran Wang a, b, c, 1, Jing Zhang a, b, Xiaoqun Wang a, b, Binxu Sun a, b, Xian Xiao a, b, c, Haojian Zhang a, b, c, Yanqi Song d, Yingjie Jia a, b, *
2023,15(4):485-495, DOI: https://doi.org/10.1016/j.chmed.2023.05.003
[Abstract] (25) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Prostate cancer remains the second most common malignancy in men worldwide, is a global health issue, and poses a huge health burden. Precision medicine provides more treatment options for prostate cancer patients, but its popularity, drug resistance, and adverse reactions still need to be focused on. Chinese herbal medicines (CHMs) have been widely accepted as an alternative therapy for cancer, with the advantages of multiple targets, multiple pathways, and low toxicity. We searched the experimental research and clinical practice of CHMs for prostate cancer treatment published in PubMed, Embase, and Web of Science in the last five years. We found five CHM formulas and six single CHM extracts as well as 12 CHM-derived compounds, which showed induction of apoptosis, autophagy, and cell cycle arrest, suppression of angiogenesis, proliferation, and migration of prostate cancer cells, reversal of drug resistance, and enhancement of anti-tumor immunity. The mechanisms of action include the PI3K/Akt/mTOR, AR, EGFR and Wnt/β-catenin signaling pathways, which are commonly implicated in the development of prostate cancer. We also summarized the advantages of CHMs in patients with hormone-sensitive and castration-resistant prostate cancer and provided ideas for their further experimental design and application.
Pharmacodynamic advantages and characteristics of traditional Chinese medicine in prevention and treatment of ischemic stroke
Hanyu Zhang a, b, Bowen Jin a, b, Xinyu You a, b, Pengrong Yi a, b, Hong Guo a, b, Lin Niu a, b, Qingsheng Yin a, b, Jiangwei Shi c, d, Yanjun Zhang c, d, *, Pengwei Zhuang a, b, *
2023,15(4):496-508, DOI: https://doi.org/10.1016/j.chmed.2023.09.003
[Abstract] (16) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Ischemic stroke (IS) is a severe cerebrovascular disease with a high incidence, mortality, and disability rate. The first-line treatment for IS is the use of recombinant tissue plasminogen activator (r-tPA). Regrettably, numerous patients encounter delays in treatment due to the narrow therapeutic window and the associated risk of hemorrhage. Traditional Chinese medicine (TCM) has exhibited distinct advantages in preventing and treating IS. TCM enhances cerebral microcirculation, alleviates neurological disorders, regulates energy metabolism, mitigates inflammation, reduces oxidative stress injuries, and inhibits apoptosis, thereby mitigating brain damage and preventing IS recurrence. This article summarizes the etiology, pathogenesis, therapeutic strategies, and relationship with modern biology of IS from the perspective of TCM, describes the advantages of TCM in the treatment of IS, and further reviews the pharmacodynamic characteristics and advantages of TCM in the acute and recovery phases of IS as well as in post-stroke complications. Additionally, it offers valuable insights and references for the clinical application of TCM in IS prevention and treatment, as well as for the development of novel drugs.
Traditional Chinese medicines for non-small cell lung cancer: Therapies and mechanisms
Fanming Kong a, b, 1, Chaoran Wang a, b, c, 1, Linlin Zhao a, b, Dongying Liao a, b, c, Xiaoqun Wang a, b, Binxu Sun a, b, Peiying Yang a, b, Yingjie Jia a, b, *
2023,15(4):509-515, DOI: https://doi.org/10.1016/j.chmed.2023.05.004
[Abstract] (17) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
The most common subtype of lung cancer is non-small cell lung cancer (NSCLC), which has a poor prognosis and seriously threatens the health of human beings. The multidisciplinary comprehensive treatment model has gradually become the mainstream of NSCLC treatment. Traditional Chinese medicine (TCM) can be used effectively either as an adjunctive therapy or alone throughout the NSCLC therapy, which has a significant impact on survival, quality of life, and reduction of toxicity. Therefore, this paper reviewed the theoretical basis, the latest clinical application, and combined treatment mechanisms in order to explore the advantage stage of TCM treatment and the synergistic therapeutic mechanisms.
Effects and mechanisms of traditional Chinese medicines on functional dyspepsia: A review
Bo Liu a, Zixiang Kou b, *, Baogui Chen b, *
2023,15(4):516-525, DOI: https://doi.org/10.1016/j.chmed.2023.06.001
[Abstract] (12) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Functional dyspepsia (FD) is a common and frequently occurring disease in clinic. With the influence of environmental factors, social factors and dietary factors, the incidence rate of FD in the general population is yearly increasing. Traditional Chinese medicine has a long history and far-reaching influence in the treatment of FD. It can prevent and treat FD in the form of multiple-components, targets and channels, with obvious effect and prominent advantages. This article starts with the common syndrome types of FD, and discusses the research progress of single Chinese medicine, effective ingredients and the mechanism of traditional Chinese medicines in treating FD, in order to provide a theoretical basis for the treatment of FD with traditional Chinese medicines.
Therapeutic effects of Chinese herbal medicines for treatment of urolithiasis: A review
Chen Jiang a, Lingyun Wang b, Yang Wang a, Rongjia Xu a, Hongtao Yang a, *, Jibin Peng b, c, *
2023,15(4):526-532, DOI: https://doi.org/10.1016/j.chmed.2023.09.001
[Abstract] (11) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Urolithiasis is a common and complex disease of the urinary system, which can cause urinary tract blockage, urinary tract infection, and even damage to urinary system-related tissues. Although urolithiasis can be cured, its high recurrence rate and the development of chronic kidney disease in some patients have drawn the attention of nephrologists. Although the application of extracorporeal lithotripsy, percutaneous nephrolithotomy and other minimally invasive techniques have made the treatment of urolithiasis more efficient, pharmacotherapy plays an indispensable role in reducing their morbidity and recurrence rates. Traditional Chinese medicine (TCM) has been used for treatment and prevention of urolithiasis in developing countries for centuries, known for its unquestionable efficacy and safety. This article reviews the progress of clinical trials and pharmacological studies on the treatment of urolithiasis with Chinese herbal medicines (CHMs). The mechanism of CHMs in the treatment of urolithiasis mainly involve preventing further growth and aggregation of urolithiasis, reducing the PH of urine, promoting calculus dissolution. Furthermore, some CHMs can increase urine output, relax smooth muscles, and promote the removal of calculus. These findings provide new treatment strategies and options for urolithiasis and secondary kidney damage.
Original articles
Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro
Kehinde Oluseun Sodeinde a, *, Akinwunmi Oluwaseun Adeoye b, *, Adedeji Adesipo a, Adebayo A. Adeniyi a, d, John Adeolu Falode b, Tajudeen Olabisi Obafemi c, Samuel Olalekan Olusanya a, Linette Twigge d, Jeanet Conradie d, Timothy O. Mosaku e
2023,15(4):533-541, DOI: https://doi.org/10.1016/j.chmed.2023.01.006
[Abstract] (15) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol (with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore (mPTP). Methods: The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions. Results: 1H and 13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders. Conclusion: Reduction in the activity of calcium ATPase and the expression of Caspase-3 and-9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.
Scutellarin alleviates liver injury in type 2 diabetic mellitus by suppressing hepatocyte apoptosis in vitro and in vivo
Xiaoming Fan a, *, 1, Yiyu Wang b, c, 1, Xifan Li a, Taiqing Zhong b, Chunlan Cheng b, Yunfei Zhang d, *
2023,15(4):542-548, DOI: https://doi.org/10.1016/j.chmed.2023.03.007
[Abstract] (13) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: Scutellarin is a primary active composition come from Erigeron breviscapus. It is well known that scutellarin has anti-inflammatory and antioxidant physiological functions. In this study, we detect the effects of scutellarin on hepatocyte cell apoptosis in type 2 diabetes mellitus (T2DM) rats. Methods: Sprague Dawley (SD) (6–8 weeks, 160–180 g) rats were randomly divided into six groups: control, model, scutellarin low-dose, medium-dose, high-dose treatment, and rosiglitazone positive groups; with 10 SD rats in each group (n = 10). The changes of biochemical factors in serum were detected by automatic biochemical instrument, the pathological changes of liver tissue were detected by hematoxylin and eosin (HE) staining, the apoptosis of liver tissue and cells was detected by tissue staining and flow analyzer, and the expression of apoptosis-related factors were determined by qPCR, Western blot and immunohistochemistry in liver tissues or cells. Results: The results showed that scutellarin decreased the levels of fasting blood glucose, total cholesterol, triglyceride, and low-density lipoprotein and increased the levels of high-density lipoprotein. Meanwhile, scutellarin decreased the levels of alanine transaminase (ALT) and aspartate transaminase (AST) and improved liver function. In addition, scutellarin suppressed the secretion of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and reduced hepatocyte apoptosis. Furthermore, scutellarin inhibited the expression of cleaved Caspase-3, Bax, and cytochrome C (Cyt-C) and promoted the expression of Bcl-2. Conclusion: Scutellarin can inhibit the apoptotic pathway, thereby relieving T2DM.
Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies
Shweta Jain a, Shalini Tripathi b, Pushpendra Kumar Tripathi c, *
2023,15(4):549-555, DOI: https://doi.org/10.1016/j.chmed.2023.02.007
[Abstract] (17) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: To extract and isolate berberine from Berberis aristata (Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed. Methods: The berberine was isolated from B. aristata using microwave-assisted extraction (MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund’s adjuvant (CFA)-induced arthritis rat model. Results: The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinflammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue inflammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with berberine. Conclusion: The ameliorating action on haematological parameters and proinflammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.
Mangiferin alleviates renal inflammatory injury in spontaneously hypertensive rats by inhibiting MCP-1/CCR2 signaling pathway
Xiaoqin Hu a, b, c, 1, Wei Zhao a, 1, Jiagang Deng a, b, Zhengcai Du a, Xuewen Zeng a, c, *, Bei Zhou a, b, Erwei Hao a, b
2023,15(4):556-563, DOI: https://doi.org/10.1016/j.chmed.2022.12.008
[Abstract] (9) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: Hypertension is a low-grade inflammation state of the disease and was easily complicated by kidneys’ inflammatory response. Mangiferin (MGF), a pharmacologically active compound in various plants including Mangifera indica, has a strong anti-inflammatory activity. However, the effects of MGF on renal inflammatory injury in spontaneously hypertensive rats (SHRs) remain unclear. The purpose of this study was to investigate the protective effects and mechanisms of MGF on renal inflammatory injury in SHRs. Methods: MGF was used in SHRs at the doses of 10, 20, 40 mg/kg/d for 8 weeks consecutively. The blood and urine were collected for assessment of renal function. Renal tissues were collected for histological, immunohistochemistry, ELISA, Western blot and real time reverse transcription PCR (RT-PCR) analysis. Results: The results showed that the levels of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and recombinant chemokine C-C-Motif receptor 2 (CCR2) were increased in SHRs, meanwhile, the level of IL-10 was decreased in SHR. Treatment of MGF inhibited the expression of IL-6, TNF-a, MCP-1 and CCR2, and promoted the expression of IL-10. Furthermore, the content of blood urea nitrogen (BUN) and serum uric acid (SUA) was significantly increased in the model group, and treatment of MGF had no obvious effects on these parameters at all dose levels. Conclusion: Our study proved that the kidneys of SHRs had significant inflammatory injury, and MGF had the protective effects on renal inflammatory injury in SHRs; The protective mechanism may be mediated partly by the MCP-1/CCR2 signaling pathway. Thus, it is a potential new drug for the treatment of hypertension.
Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem
Yanan Yang a, Xiaohui Zhao b, Yong Xie a, *, Chongming Wu b, *
2023,15(4):564-573, DOI: https://doi.org/10.1016/j.chmed.2023.02.003
[Abstract] (14) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi (PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions. However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largelym unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them. Methods: Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively. Results: Oral administration of PAL notably enriched anti-inflammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-inflammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus, Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups (groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum, Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp. Conclusion: Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.
Transcriptome analysis of Saposhnikovia divaricata and mining of bolting and flowering genes
Min Zhang a, b, 1, Wenle Wang a, c, 1, Qian Liu b, Erhuan Zang b, Lijun Wu d, Guofa Hu d, Minhui Li a, b, c, *
2023,15(4):574-587, DOI: https://doi.org/10.1016/j.chmed.2022.08.008
[Abstract] (4) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: Early bolting of Saposhnikovia divaricata has seriously hindered its medicinal value and sustainable development of resources. The molecular mechanism of bolting and flowering of S. divaricata is still unclear and worth of research. In our study, we explored the transcriptome of the genes related to the bolting and flowering of S. divaricata. Methods: The transcriptome library was constructed, sequenced, assembled and annotated from the bolting and unbolting leaves of S. divaricata by high-throughput sequencing at the bud and flowering stage. Focus on the pathways related to bolting and flowering in plants, and explore genes. The expression of seven candidate genes was verified by real-time fluorescence quantitative PCR (qRT-PCR). Results: Transcriptome results showed that 249 889 422 high-quality clean reads were obtained. A total of 67 866 unigenes were assembled with an average length of 948.1 bp. Trinity de Novo assembly produced 67 866 unigenes with an average length of 948.1 bp. Among 993 differentially expressed genes, 484 genes were significantly up-regulated and 509 genes were down-regulated in the SdM group. A total of 79 GO terms were significantly enriched for differentially expressed genes. KEGG results showed that 11 154 unigenes were enriched in 89 pathways. And 21 candidate genes related to bolting and flowering of S. divaricata were excavated. The qRT-PCR results showed that expression trends of HDA9, PHYB, AP2, TIR1, Hsp90, CaM, and IAA7 were consistent with transcriptomic sequencing results. In addition, RNA-seq had identified 10 740 transcription factors and classified them into 58 families by their conserved domains. Further studies showed that the transcription factors regulating the flowering of S. divaricata were mainly distributed in the NAC, MYB_related, HB-other, ARF, and AP2 families. Conclusion: Based on the results of this study, it was found that the plant hormone signal transduction pathway was one of the decisive factors to control bolting and flowering. Among them, auxin related genes IAA and TIR1 are the key genes in the bolting and flowering process of S. divaricata.
Chinese yam yield is affected by soil nutrient levels and interactions among N, P, and K fertilizers
Yang Chen a, 1, Xianzhi Zhou a, b, 1, *, Lina Ma a, Yongsheng Lin a, Xiangui Huang a
2023,15(4):588-593, DOI: https://doi.org/10.1016/j.chmed.2022.11.006
[Abstract] (10) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: The current fertilization methods for Chinese yam are uneconomic and unfriend to environment. A rational one is very important to achieve desired balance of high yield of Chinese yam, economic and friend to environment. Here, we studied the effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizers on the yield of ‘Qinfeng’ Chinese yam in shallow-groove directional cultivation. Methods: The experiments were conducted in Dehua County, Fujian Province, China using a ‘‘3414” optimal design. Overall, three fertilizer factors (N, P, and K) were evaluated at the following four levels: 0, no fertilizer; 1, 0.5-fold the typical rate; 2, typical fertilization rate; and 3, 1.5-fold the typical rate. There were 14 different fertilization treatments. Results: Treatment 6 (N2P2K2) produced the longest (75.6 cm) and thickest tubers (4.9 cm) with the highest tuber fresh weight (1311.9 g) and yield (41,015.9 kg/hm2), whereas, treatment 1 produced the shortest (65.6 cm) and thinnest tubers (3.9 cm) with the lowest fresh weight (953.4 g) and yield (28 532.8 kg/hm2) among the 14 fertilizer combinations. The experimental data could be fitted to single-variable quadratic and binary quadratic models but not to a ternary quadratic polynomial model. Appropriate N, P, and K fertilizer application rates increased Chinese yam yield. However, excessive fertilization lowered the yield. Chinese yam yield was significantly and strongly correlated with the amounts of N, P, and K fertilizer applied. Conclusion: Based on the single variable quadratic and binary quadratic models, we propose that the quantities of N, P, and K fertilizer used to grow 1 hm2 ‘Qinfeng’ Chinese yam should be 360–388.3, 90–100.95, and 416.3–675 kg, respectively.
Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures
Wenwen Yuan a, Weihua Yuan a, Rong Zhou a, Guoying Lv b, Mengni Sun a, Yanxia Zhao a, *, Weifa Zheng a, *
2023,15(4):594-602, DOI: https://doi.org/10.1016/j.chmed.2022.07.004
[Abstract] (16) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: The medicinal mushroom Sanghuangporus vaninii produces pharmaceutically valuable hispidin polyphenols in natural habitats. However, due to the slow growth in nature, S. vaninii grown in the field (sclerotia) is not reliable for pharmaceutical purposes. Although higher biomass of fungal mycelia can be obtained in submerged cultures, the accumulation of hispidin polyphenols is rare. Methods: In this study, the polyunsaturated fatty acids (PUFAs), linoleic acid (LA), linolenic acid (ALA), and methyl jasmonate (MeJa) were employed as the stimulant agents to coordinate the accumulation of biomass and hispidin polyphenols in its submerged cultures. Results: The addition of LA and ALA promoted the mycelial accumulation, while the addition of MeJa inhibited the growth of S. vaninii concomitant with reduced total polyphenols. UPLC-Triple-TOF-MS analysis revealed an increased production of hispidin, phellinstatin, pinnilidine, and its derivatives upon the addition of LA and ALA, and hypholomine B and its isomer, 3,14’-bihispidinyl, and phelligridin E upon the addition of MeJa on day 13. Intriguingly, total polyphenols from the MeJa-supplementing cultures harbored a high capacity in scavenging free radicals. Chemical structural analysis showed that hispidin polyphenols had higher antioxidant activity due to more hispidin moieties induced by MeJa. Conclusion: The supplement of PUFAs affects the synthesis and composition of hispidin polyphenols in S. vaninii. Our results provide a possibility to coordinate the production of hispidin polyphenols via submerged cultures of S. vaninii.
Two new phenolic amides from Allium chinense
Xiaoqing Li a, 1, Shikai Yan a, 1, Jihong Lu a, Rui Wang a, b, Xianpeng Ma c, Xue Xiao b, *, Yan Zhang a, *, Huizi Jin a, *
2023,15(4):603-606, DOI: https://doi.org/10.1016/j.chmed.2022.12.006
[Abstract] (19) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: To isolate the phenolic amides from the dried bulbs of Allium chinense and investigate their myocardium protective activities. Methods: The chemical constituents were isolated and purified by combining with silica gel column, Sephadex LH-20 column, HPLC and other chromatography techniques. Their structures were elucidated by NMR techniques and mass spectrometry. The isolated compounds were evaluated to determine their protective effect for myocardium cells in vitro. Results: Two new phenolic amides, namely, alichinemide I (1) and alichinemide II (2), and six konwn amides were isolated from the dried bulbs of A. chinense. The structures of compounds 3–8 were identified as 3-indolcarbaldehyde (3), 1-(2-aminophenyl)urea (4), 2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole3-carboxylic acid (5), N-trans-feruloyltyramine (6), N-trans-p-coumaroyltyramine (7), and N-(3,4-dimethoxyphenethyl) acetamide (8). Compound 3 (50 lmol/L) showed significant inhibitory effect on the damage of H9c2 myocardial cells induced by H2O2 in vitro. Conclusion: Compounds 1 and 2 were new phenolic amides. Compound 3 could be one of the potential myocardium protective constituents of A. chinense.
Comparative quantitative analysis of fruit oil from Hippophae rhamnoides (seabuckthorn) by qNMR, FTIR and GC–MS
Dattatraya Dinkar Gore a, Furkan Ahmad a, Kulbhushan Tikoo a, Arvind Kumar Bansal a, Dinesh Kumar b, Inder Pal Singh a, *
2023,15(4):607-613, DOI: https://doi.org/10.1016/j.chmed.2023.05.005
[Abstract] (12) [HTML] (0) [PDF 0.00 Byte] (0)
Abstract:
Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides (seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and FTIR. Methods: SBT fruit oil (IPHRFH) was extracted with hexane and the triglyceride (TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers (Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR spectroscopy. Results: The qNMR results showed that the oil contained 80.3% of triacylglycerol (TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier (SW) were fruit oil; All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed oil. Conclusion: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore, analysis of commercial samples showed discrepancies between label claims and actual content.

Select AllDeselectExport
Display Method:
Pharmacopoeia of the People’s Republic of China (2010 Edition): A Milestone in Development of China’s Healthcare
QIAN Zhong-zhi, DAN Yang, LIU Yan-ze, PENG Yong
2010,2(2):157-159, DOI: 10.3969/j.issn.1674-6384.年份.期.第几篇文章
[Abstract] (4767) [HTML] (0) [PDF 217.57 K] (23653)
Abstract:

Feature Extraction of Chinese Materia Medica Fingerprint Based on Star Plot Representation of Multivariate Data
CUI Jian-xin, HONG Wen-xue, ZHOU Rong-juan, GAO Hai-bo
2011,3(2):140-143, DOI: 10.3969/j.issn.1674-6384.2011.02.009
[Abstract] (2167) [HTML] (0) [PDF 142.79 K] (19339)
Abstract:
Objective To study a novel feature extraction method of Chinese materia medica (CMM) fingerprint. Methods On the basis of the radar graphical presentation theory of multivariate, the radar map was used to figure the non-map parameters of the CMM fingerprint, then to extract the map features and to propose the feature fusion. Results Better performance was achieved when using this method to test data. Conclusion This shows that the feature extraction based on radar chart presentation can mine the valuable features that facilitate the identification of Chinese medicine.
Analysis on Volatile Constituents in Leaves and Fruits of Ficus carica by GC-MS
LI Jun, TIAN Yu-zeng, SUN Bao-ya, YANG Dan, CHEN Ji-ping, MEN Qi-ming
2012,4(1):63-69, DOI: 10.3969/j.issn.1674-6384.2012.01.010
[Abstract] (1970) [HTML] (0) [PDF 160.29 K] (12232)
Abstract:
Objective To identify and analyze the volatile constituents in the leaves and fruits of Ficus carica. Methods Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were used. Results The major components detected in volatile oil of the leaves were psoralen (10.12%), β-damascenone (10.17%), benzyl alcohol (4.56%), behenic acid (4.79%), and bergapten (1.99%), etc. The major components detected in volatile oil of the fruits were furfural (10.55%), 5-methyl-2-furaldehyde (10.1%), and benzeneacetaldehyde (6.59%), etc. Conclusion A total of 121 volatile constituents are identified in the leaves and 108 in the fruits of F. carica, among which 103 constituents are identified for the first time in the leaves and 100 in the fruits. Eighteen volatile constituents are identified in both leaves and fruits.
New Collection of Crude Drugs in Chinese Pharmacopoeia 2010 I. Callicarpa Linn. and Related Items
DAN Yang, QIAN Zhong-zhi, LIU Yan-ze, ZHOU Guo-ping, PENG Yong, XIAO Pei-gen
2010,2(4):272-288, DOI: 10.3969/j.issn.1674-6384.2010.04.005
[Abstract] (3694) [HTML] (0) [PDF 307.65 K] (11148)
Abstract:
Callicarpa Linn. (beautyberry) is one of the major genera in Verbenaceous, about 20 of which are medicinal plants. Beautyberry, called Zizhu in China, is a generic name of those species and largely used as hemostatic medicine. The Chinese Pharmacopoeia 2010 has admitted three new crude drugs from the genus of Callicarpa Linn. including Callicarpae Macrophyllae Folium, Callicarpae Caulis et Folium, and Callicarpae Formosanae Folium for the first time since the 1977 version of the Chinese Pharmacopoeia. In order to better understand these new crude drugs, we systematically described their bibliography, admission reasons, botanical identification, chemistry, and pharmacology. Several other species, out of national regulations but intensively studied and widely used, are also covered in this review.
HPLC Fingerprint and LC-TOF-MS Analysis on Extract from Roots of Gentiana macrophylla
SU Qi, SHANG Ping-ping, ZHANG Yong-min, JIA Na, HE Jiao, ZHAO Wen-na, SUN Wen-ji
2012,4(3):245-251, DOI: 10.3969/j.issn.1674-6384.2012.03.012
[Abstract] (2705) [HTML] (0) [PDF 221.06 K] (11054)
Abstract:
Objective Establishing a ?ngerprint method to identify the characteristic chemicals in the roots of Gentiana macrophylla and evaluate their quality. Methods RP-HPLC was developed for ?ngerprint analysis and determination of four ingredients in G. macrophylla roots from different sources. LC-ESI-TOF-MS was employed to identify the chromatographic peaks of the ?ngerprint. Results Five common peaks were identified by comparing their retention time with reference secoiridoid glucosides. Eight major peaks in chromatographic fingerprint were analyzed by on-line LC-ESI-TOF-MS. Four secoiridoid glucosides were identified based on their MS data. Conclusion The method is specific and could be served for the quality identi?cation and comprehensive evaluation of G. macrophylla.
Network Pharmacology Bridges Traditional Application and Modern Development of Traditional Chinese Medicine
Chang-xiao Liu, Rui Liu, Hui-rong Fan, Xue-feng Xiao, Xiao-peng Chen, Hai-yu Xu, Yan-ping Lin
2015,7(), DOI:
[Abstract] (2528) [HTML] (0) [PDF 503.58 K] (11040)
Abstract:
Traditional Chinese medicine (TCM) has developed over thousands of years and has accumulated abundant clinical experience, forming a comprehensive and unique medical system. Emerging evidence has begun to illustrate TCM as an area of important medical rediscoveries. This paper briefly introduced the concept, significance, and technology of network pharmacology based on network biology and systems biology. It focused on the theoretical system and potential prospect of TCM network applied in TCM research and development including predicting new drug targets, action mechanism, new drug discovery; evaluating pharmacodynamics, pharmacokinetics, safety, toxicology, quality control, and bioinformatics of drugs. We also discussed the opportunities and challenges in the development and application of network pharmacology in the modernization of TCM research.
Review of Rhubarbs: Chemistry and Pharmacology
ZHENG Qing-xia, WU Hai-feng, GUO Jian, NAN Hai-jiang, CHEN Shi-lin, YANG Jun-shan, XU Xu-dong
2013,5(1):9-32, DOI: 10.7501/j.issn.1674-6384.2013.01.003
[Abstract] (2634) [HTML] (0) [PDF 583.26 K] (10697)
Abstract:
Rhubarb is a perennial herb belonging to the genus Rheum L. (Polygonaceae). Rhei Radix et Rhizoma (rhubarb roots and rhizomes) is one of the most popular Chinese materia medica and has been widely used for strong laxative function. About 200 compounds with six different types of skeletons (anthraquinone, anthrone, stilbene, flavonoids, acylglucoside, and pyrone) have so far been isolated from eighteen species of the genus Rheum L. These constituents showed extensive pharmacological activities including cathartic, diuretic, anticancer, hepatoprotective, anti-inflammatory, and analgesic effects, as well as toxicological effects. Chemical fingerprint, LC-MS, and other analytical techniques have been used for the quality control of rhubarb. This comprehensive review summarizes the researches into the isolation, pharmacological activities, and phytochemical analysis reported since investigations began in the late 1940s. In addition, pharmacokinetic studies and clinical application of rhubarb are also discussed in present paper.
Revision and Improvement of Criterion on Traditional Chinese Medicines in Chinese Pharmacopoeia 2015
Yang Dan1, Zhong-zhi Qian2, Yong Peng1, Chang-qing Chen3, Yan-ze Liu1, Wen Tai3, Jing-wen Qi3
2016,8(3):196-208, DOI: 10.1016/S1674-6384(16)60043-6
[Abstract] (1136) [HTML] (0) [PDF 311.21 K] (10285)
Abstract:
Chinese Pharmacopoeia is updated every five years, of which traditional Chinese medicine (TCM) is the most important part. The 2015 version completed by the 10th Pharmacopoeia Commission has come into operation since December 1, 2015. Here we introduced the revision and improvement of quality evaluation and control standards of TCMs in Chinese Pharmacopoeia 2015.
Authentication of Medicinal Plants by DNA Based Markers and Genomics
HAO Da-cheng, CHEN Shi-lin, XIAO Pei-gen, PENG Yong
2010,2(4):250-261, DOI: 10.3969/j.issn.1674-6384.2010.04.003
[Abstract] (3163) [HTML] (0) [PDF 401.07 K] (9415)
Abstract:
For the protection of consumers and developments of relevant industry, authentication of medicinal plants is a critical issue. This review covers various aspects of authentication methods and techniques based on molecular biology and genomics with special emphasis on molecular biology techniques including genome-based authentication, microchip-based authentication, DNA barcoding, and their applications.
Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography
WAN Jin-zhi, CHEN Xin-xia, QIU Chun-mei, LIN Xiang-cheng
2010,2(2):148-152, DOI: 10.3969/j.issn.1674-6384.2010.02.010
[Abstract] (3993) [HTML] (0) [PDF 215.71 K] (9370)
Abstract:
Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production. Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A. vera. The biphasic solvent system composed of hexane-ethyl acetate-acetone-water (0.2︰5︰1.5︰5) was used at a flow rate of 1.0 mL/min, while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min. The effluent was detected at 254 nm. Results Isoaloeresin D (53.1 mg) and aloin (106.9 mg) were separated from the crude extract (384.7 mg) with the purities of 98.6% and 99.5%, respectively. Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.
Challenges in Research and Development of Traditional Chinese Medicines
LIU Chang-xiao, XIAO Pei-gen, PENG Yong, SONG Nai-ning
2009,1(1):1-28, DOI: 10.3969/j.issn.1674-6384.年份.期.第几篇文章
[Abstract] (4010) [HTML] (0) [PDF 469.82 K] (9280)
Abstract:
This review is briefly to recall the history of research and development (R&D) of Chinese materia medica (CMM) and to discuss the challenges of Chinese traditional and herbal medicines (CTHM) facing the modern science and technology. The R&D of CTHM is thought to be an important pathway for new drug discovery. Since1949, about 140 approved new drugs have been developed, among which about 80 originated directly or indirectly from medicinal plants. CTHM has gained interest from the international medical, biomedical, and pharmaceutical institutions as a valuable source of potential medicines. For the modernization of CMM and innovative research of CTHM, there are following challenges to be faced: (1) to evaluate the efficacy, pharmacological properties, action mechanism, and active chemical constituents; (2) to develop new methodologies for the quality and safety of CTHM; (3) to apply new “-omics” techniques to accelerate drug discoveries developed from CTHM; and (4) to apply international practices including good agricultural practice, good manufacturing practice, good laboratory practice, and good clinical practice in the R&D of CTHM.
Analgesic and Anti-inflammatory Effects of Ginger Oil
JIA Yong-liang, ZHAO Jun-ming, ZHANG Lin-hui, SUN Bao-shan, BAO Meng-jing, LI Fen-fen, SHEN Jian, SHEN Hui-jun, ZHAO Yu-qing, XIE Qiang-min
2011,3(2):150-155, DOI: 10.3969/j.issn.1674-6384.2011.02.011
[Abstract] (3151) [HTML] (0) [PDF 157.86 K] (8894)
Abstract:
Objective Ginger (Zingiber officinale) is widely used as a spice in cooking and as a medicinal herb in traditional herbal medicine. The present study was to investigate the analgesic and anti-inflammatory activities of ginger oil in experimental animal models. Methods The analgesic effect of the oils was evaluated by the “acetic acid” and “hot-plate” test models of pain in mice. The anti-inflammatory effect of the oil was investigated in rats, using rat paw edema induced by carrageenan, adjuvant arthritis, and vascular permeability induced by bradykinin, arachidonic acid, and histamine. Indomethacin (1 mg/kg), Aspirin (0.5 g/kg) and Dexamethasone (2.5 mg/kg) were used respectively as reference drugs for comparison. Results The ginger oil (0.25－1.0 g/kg) produced significant analgesic effect against chemically- and thermally-induced nociceptive pain stimuli in mice (P < 0.05, 0.01). And the ginger oil (0.25－1.0 g/kg) also significantly inhibited carrageenan-induced paw edema, adjuvant arthritis, and inflammatory mediators-induced vascular permeability in rats (P < 0.05, 0.001). Conclusion These findings confirm that the ginger oil can be used to treat pain and chronic inflammation such as rheumatic arthritis.
Research Progress on Chemical Constituents of Cistanches Herba and their Pharmacological Effects
Lin–lin Wang, Hui Ding, Yao Shi, Qing-hai Lai, He–shui Yu, Li-juan Zhang, Xin-bo Song
2015,7(), DOI:
[Abstract] (2167) [HTML] (0) [PDF 273.72 K] (8621)
Abstract:
Cistanches Herba, a famous traditional Chinese materia medica, has a laudatory title “desert ginseng”. According to Chinese Pharmacopoeia 2000, Cistanches Herba is the dry fleshy stem with scale-like leaves of Cistanche deserticola. In order to expand the drug source, Chinese Pharmacopoeia 2005 increased the plants in Cistanche Hoffmg. et Link (Orobanchaceae), C. tubulosa, and dry scaly leaf succulent stems were as genuine Cistanches Herba and still in use. In order to have more comprehensive understanding on Cistanches Herba, this paper focuses the reviews on the biological characteristics, chemical constituents, and pharmacological activities of Cistanches Herba, and its development prospects are looked forward. It provides the relative theoretical basis for the subsequent research work of Cistanches Herba and can be conducive to the development and utilization of Cistanches Herba.
Concentration Prediction of Total Flavonoids in Aurantii Fructus Extraction Process: Locally Weighted Regression versus Kinetic Model Equation Based on Ficks Law
Yang Chen, Jun-hui Shen, Jian Ni, Meng-jie Xu, Hao-ran Dou, Jing Fu, Xiao-xu Dong
2015,7(), DOI:
[Abstract] (1967) [HTML] (0) [PDF 324.52 K] (8495)
Abstract:
Objective To predict the total flavonoids concentration of Aurantii Fructus fried with bran in its extraction process. Methods Ultraviolet spectrophotometry was used to determine the concentration of total flavonoids in different extraction time (t ) and solvent load (M ). Then the predicted procedure was carried out using the following data: 1) based on Ficks second law, the parameters of the kinetic model could be deduced and the equation was established; 2) Locally weighted regression (LWR) code was developed in the WEKA software environment to predict the concentration. And then we used both methods to predict the concentration of total flavonoids in new experiments. Results After comparing the predicted results with the experimental data, the LWR model had better accuracy and performance in the prediction. Conclusion LWR is applied to analyze the extraction process of Chinese herb for the first time, and it is totally fit for the extraction. LWR-based system is a more simple and accurate way to predict than the established equation. It is a good choice especially for a process which exists no clearly rules, and can be used in the real-time control during the process.
Bioactivities and Pharmaceutical Effects of 1-Aminocyclopro- panecarboxylic Acid
ZHU Qin-wei, KRAEMER Alexander, ZHU Xu-xiang
2010,2(2):81-105, DOI: 10.3969/j.issn.1674-6384.2010.02.001
[Abstract] (3265) [HTML] (0) [PDF 822.05 K] (8144)
Abstract:
Objective The 1-aminocyclopropanecarboxylic acid (ACPC) is a natural micromolecule non-protein amino acid that exists only in plants. Despite the determination of its regulating effects on plants, several bioactivities and effects on animals or humans are still unclear. This review focuses the pharmaceutical effects，above all, the neuroprotective effects of ACPC on the cerebro- and cardiovascular system. Methods Two hundred and seventy nine studies were selected and identified from a total of 2457 references in Medline and Chemical Abstracts from 1957 to 2008. Only 38 articles on neuroprotective effects of ACPC from seven different countries qualified to be included in the analysis. Results ACPC has altogether following six general neuroprotective effects on the brain and nervous system: anti-neurotoxicity induced by NMDA, dynorphin-A, glutamate, and morphine; protection from cerebral neurological injury induced by ischemia; antidepressant and anxiolytic effects; anti-convulsion and -seizures and control of epilepsy; facilitation of spatial learning and memory; and the effect of reducing alcohol consumption. Conclusion ACPC has shown a variety of effects on plants and animals. The bioactivities and pharmaceutical effects on animals are of great significance to medical research and public health. Further clinical trials or epidemiological studies are needed to determine its effect in humans. Food intervention with ACPC-rich vegetables and fruits may be a suitable therapy for cerebro- and cardiovascular system diseases. Administration of trace exogenous ACPC could produce vegetables and fruits of rich endogenous ACPC.
Chemical and Pharmacological Researches on Hyoscyamus niger
LI Jun, SHI Ji, YU Xin-wen, SUN Jing-kuan, MEN Qi-ming, KANG Ting-guo
2011,3(2):117-126, DOI: 10.3969/j.issn.1674-6384.2011.02.006
[Abstract] (4158) [HTML] (0) [PDF 211.05 K] (8074)
Abstract:
The reports on chemical constituents of Hyoscyamus niger were summarized. The compounds include alkaloids, saponins, lignans, coumarinolignans, flavonoids, and some other nonalkaloidal compounds. TLC, HPLC, and GC were used for the qualitative and quantitative analyses of some chemical constituents in H. niger. Modern pharmacological experiments showed that H. niger had the analgesic, anti-inflammatory, antipyretic, anticonvulsant, spasmolytic, antidiarrhoeal, antisecretory, bronchodilatory, urinary bladder relaxant, hypotensive, cardiosuppressant, vasodilator, antitumor, and feeding deterrent properties. In addition, the toxicities of this medicinal plant were also described.
In silico Molecular Docking Study of Repensine and Bentysrepinine against HBV DNA Polymerase
Fan-cui Meng, Wei-ren Xu, Ya-zhuo Li, Zheng-ming Huang, Guang-yi Liang, Chang-xiao Liu
2015,7(), DOI:
[Abstract] (2193) [HTML] (0) [PDF 488.37 K] (7983)
Abstract:
Bentysrepinine (Y101), a derivative of repensine, is a novel di-peptide structure isolated from Dichondra repens. In vitro and in vivo tests exhibited that bentysrepinine markedly inhibited DNA-HBV and cccDNA activities. The binding mode of Y101 and repensine with DNA polymerase was driven by hydrophobic interactions. This might provide novel recognition of inhibitory effect of Y101 against HBV, though its inhibition mechanism needs to be validated by bio-assay at cellular level and of polymerase activity. Preliminary docking study suggested that Y101 might be able to inhibit HIV inverse transcriptase, also have the potential to interact with DNA polymerase and HCV NS5B polymerase.
Network Pharmacology for Traditional Chinese Medicine Research: Methodologies and Applications
Yu-feng Liu, Ni Ai, Anthony Keys, Xiao-hui Fan, Min-jun Chen
2015,7(), DOI:
[Abstract] (1953) [HTML] (0) [PDF 299.63 K] (7794)
Abstract:
The inception of network pharmacology comes from the advance in “multi-target, multi-drug” paradigm and opens up a new field for pharmaceutical science. Traditional Chinese medicine (TCM) is well-known for its use of medicinal herb combinations to treat the functional disorders induced by diseases through a holistic view, which naturally followed the principal of network pharmacology. In this review, the methodologies of network pharmacology in TCM studies were summarized. Specifically, the methodologies for network construction and network analysis were detailed by following several TCM study cases followed. The perspectives for TCM network pharmacology were also provided.
Simultaneous Determination of Five Hydrophilic and Lipophilic Components from roots of Salvia miltiorrhiza by HPLC
Li-lan Lu, Shuang Hou, Ting-ting Zheng, Xing-li Zhang, Jian-he Wei
2015,7(), DOI:
[Abstract] (1858) [HTML] (0) [PDF 360.32 K] (7531)
Abstract:
Objective A reversed-phase HPLC method was established for the simultaneous determination of five hydrophilic and lipophilic components in the roots of Salvia miltiorrhiza. Methods Hydrophilic components including danshensu, protocatechuic aldehyde, and salvianolic acid B, and lipophilic components such as cryptotanshinone and tanshinone IIA, were successfully separated on a Waters Symmetry C18 reverse- phase column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.5% phosphoric acid (gradient elution) as mobile phase, the detection wavelength was set at 281 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 30 °C. Results The recovery of the method was in the range of 95.1%–102.5% and the precision was less than 3% for all five analytes. All the compounds showed good linearity (R2 > 0.9990) in a relatively wide concentration range. Therefore, this HPLC method demonstrated good reproducibility, stability, and accuracy in validation studies. Conclusion Simultaneous quanti?cation of the multiple components by HPLC would be a better strategy for the quality evaluation on the roots of S. miltiorrhiza.
Reflecting the Steps of Modernization of Chinese Materia Medica from the Papers in Chinese Traditional and Herbal Drugs in 2009
LIU Yan-ze, DAN Yang, PENG Yong, XIAO Pei-gen
2010,2(3):180-188, DOI: 10.3969/j.issn.1674-6384.2010.03.004
[Abstract] (2251) [HTML] (0) [PDF 265.22 K] (7247)
Abstract:
The publications on the journal of Chinese Traditional and Herbal Drugs in Vol.40, 2009 are briefly reviewed in the categories of chemical constituents, preparations and technologies, analysis and quality control, pharmacological and clinical studies, reviews, and finally healthy principles. Some comments, especially for hot topics have been personally provided.