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  • 1  Feature Extraction of Chinese Materia Medica Fingerprint Based on Star Plot Representation of Multivariate Data
    CUI Jian-xin HONG Wen-xue ZHOU Rong-juan GAO Hai-bo
    2011, 3(2):140-143. DOI: 10.3969/j.issn.1674-6384.2011.02.009
    [Abstract](1157) [HTML](0) [PDF 142.79 K](2115) [Cited by](1)
    Abstract:
    Objective To study a novel feature extraction method of Chinese materia medica (CMM) fingerprint. Methods On the basis of the radar graphical presentation theory of multivariate, the radar map was used to figure the non-map parameters of the CMM fingerprint, then to extract the map features and to propose the feature fusion. Results Better performance was achieved when using this method to test data. Conclusion This shows that the feature extraction based on radar chart presentation can mine the valuable features that facilitate the identification of Chinese medicine.
    2  Influence of Panax ginseng Continuous Cropping on Metabolic Function of Soil Microbial Communities
    YING Yi-xin DING Wan-long ZHOU Ying-qun LI Yong
    2012, 4(4):330-335. DOI: 10.3969/j.issn.1674-6348.2012.04.011
    [Abstract](922) [HTML](0) [PDF 188.29 K](1889) [Cited by](1)
    Abstract:
    Objective To investigate the influence of Panax ginseng continuous cropping on the carbon substrate metabolic activity of microbes in soils sampled from Dafang, Huangni, and Wulidi in Jilin Province, China. Methods Soil metabolisms of soil communities were characterized by community level physiological profiles using BIOLOGTM EcoPlate. Results Soils sampled from the three sites were analyzed and their metabolic activities were compared. Principal component analysis explored the significant variance in metabolic function of microbial communities in soils, though the Shannon index and the evenness index of them were similar. Futhermore, two principal components (PC1 and PC2), which contributed 67.83% and 10.78% of total variance, were extracted respectively. And also, substrates significantly correlated with PC1 and PC2 at the three sampling sites were identified. Conclusion Characteristic of soil is the primary factor influencing microbial communities, and P. ginseng continuous cropping has significant influence on microbial community. Though soil samples show similar microbial metabolic profiles, microbial communities in rhizosphere soil are changed obviously during the cultivation of P. ginseng, which would finally result in the unbalance of microbial community. Phytopathogens would gradually be the predominants in rhizosphere soil and make P. ginseng sick.
    3  Effect of n-butanol Extract from Potentilla anserina on Hypoxia- induced Calcium Overload and SERCA2 Expression of Rat Cardiomyocytes
    LI Ling-zhi WANG Lu-jun WANG Yue CUI Ying LI Jian-yu ZHANG Li ZHANG Yong-liang
    2012, 4(2):142-149. DOI: 10.3969/j.issn.1674-6384.2012.02.008
    [Abstract](1043) [HTML](0) [PDF 541.86 K](1850) [Cited by](1)
    Abstract:
    Objective To investigate the effect of n-butanol extract from Potentilla anserina (NP) intervention on hypoxia-induced Ca2+ overload and SERCA2 expression of rat cardiomyocytes. Methods Primary cultured myocardial cell from SD neonatal rat (1-3 d) was used in the establishment of hypoxia model. After hypoxia for 3 h, the Ca2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe, and the biochemical indicator intracellular Ca2+-ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum (SR) Ca2+-ATPases (SERCA2) were assayed with RT-PCR, Western-blotting, and immune-cytochemical staining in each group. Results The results showed that NP decreased Ca2+ concentration, increased the activity of Ca2+-ATPase, and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group. Conclusion These results indicate that NP could attenuate the Ca2+ overload. The mechanism might be explained as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats, and further enhance the capacity of SR Ca2+ re-uptake.

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