Volume 10,Issue 4,2018 Table of Contents

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  • 1  Understanding "medicine and food homology", developing utilization in medicine functions
    Liu Chang-xiao
    2018, 10(4):337-338.
    [Abstract](51969) [HTML](0) [PDF 0.00 Byte](8)
    Abstract:
    Chinese medicine is a very big concept of medicine. In Shen-nong era, medicine and food do not divide. "Medicine and food homology", which is called “Chinese medicine and dietary application”, refers to that many foods are drugs, and there is no absolute demarcation line between them. At that time, seventy poisons were encountered in a day for experimental accusations, herbal medicines are divided into upper, middle and lower categories, the safe and non-toxic Chinese medicines are the upper product, which can be consumed, but is just a difference in dosage. Ancient medical scientists applied the theory of "four natures" and "five tastes" of traditional Chinese medicine (TCM) into food, believing that each food also has "four natures" and "five tastes". "Medicine and food homology" means that Chinese medicine and food are of the same origin. The theory of "medicine and food homology" based on practice has existed in Chinese traditional medicine since ancient times. In ancient primitive society, people discovered the sexual taste and efficacy of various foods and medicines in the process of searching for food, realizing that many foods can be used medicinally and many medicinal materials can be used as dietary supplement. The theory holds that many foods are both food and medicine, and that they are just as effective in preventing disease as drugs. Therefore, this theory of "medicine and food homology" is also the basis of food therapy. Strictly speaking, in the TCM, drugs and food are indiscriminate. Food has fewer side effects, but drugs have more. This is another meaning of medicine and food homology. Hence, the source of Chinese medicine and food is the same. Some things can only be used to treat diseases, known as drugs, some things can only be used for diet, known as foods. The line between medicine and food is not very clear because they all have healing powers. Such as orange, hawthorn, black plum, walnuts, almonds, fennel, cinnamon, pumpkin seeds and so on, they are not only Chinese medicine with a good curative effect, but also nutritious delicious food that people often eat. The common point of TCM and food is to have a healthy function to prevent and control diseases. The difference between them is that the TCM has strong therapeutic effect, which is often called "strong drug". When the medicine is used correctly, the effect is prominent. However, the therapeutic effect of food is not as prominent and rapid as that of TCM. The important issue cannot be ignored is that medicaments will not be often taken commonly though their action is strong, and food we eat every day though food action is weak. Our daily diet, in addition to the supply of essential nutrients, will also be more or less due to the performance of the food on the balance and physiological function of the adverse or favorable impact, accumulation, from quantitative change to qualitative change, the impact becomes very obvious. In this sense, they are no less effective than TCM. Accordingly, adjusting food correctly and reasonably, with long-term adherence, can have the effect that medicaments cannot achieve. In this issue of CHM journal, we organized four articles on Lycium barbarum, Gardenia jasminoides, Taraxacum mongolicum, and Moringa oleifera (Gouqizi, Zhizi, Pugongying, and Lamu in Chinese, respectively) with medicine and food functions, focusing on the analysis and discussion of the current research status and put forward the views or opinions of authors on the development of these four Chinese medicines. The present paper on Lycium barbarum focuses on the legal plant resource and reviews the main research in medicinal field including botanical identification, ethnopharmacological functions, phytochemistry, pharmacological effects, clinic usages, and safety issues. Fruits of Gardenia jasminoides haves been used as dietary supplement and traditional Chinese medicine for thousands of years. Recent studies of Gardenia jasminoides reveal that the extracts or the active components play an essential role in oxidative stress, inflammation, hepatoprotection, neuroprotection, anti-diabetes, antitumor and so on. Here, we reviewed Gardenia jasminoides in the botanical identity, chemical ingredients, pharmacology, and pharmacokinetics, safety and toxicity evaluation, clinical application, etc. Taraxacum mongolicum has been used in TCM and dietary application, and T. officinale has been applied in medicinal and food use in other regions and cultures around the globe. Authors summarized the phytochemical constituents of dandelion (particularly from T. mongolicum and T. officinale). Recent published health benefits of dandelion, such as anti-oxidant activity, anti-inflammatory activity, blood sugar and lipids regulation and hepatoprotective activity, as well as its safety data were highlighted. The limited human clinical study and pharmacokinetics information lead to the thought that well-designed human clinical study should be the focus and opportunity for the future research area to truly understand efficacy function and health benefit of dandelion for its application in medicine and health food area. Moringa oleifera is one of the most widespread species belonging to family Moringaceae. Almost each part of this tree has enormous properties in nutrition, medicine or other industrial purposes. This review mainly introduces nutritional ingredients, bioactive compounds, applications, and potentials of this tree including leaves, seeds, pods, flowers, root barks, and gum in order to bring convenience to industries and researchers all over the world focusing on each part of this amazing plant. Moreover, the review also analyzes the current application situation of M. oleifera in China. Authors concluded that most current researches focus on the leaves and seeds of this plant, and the most concerned property of M. oleifera is the antioxidant ability. Medicine and food dual-use plants of China have a long history of application, and the medicine-food dual-use plants have both certain physiological effects and long-term edible safety. For people with potential risk of onset, healthy products developed by edible and edible dual-use plants can prevent disease occurrence and maintain health, and are more scientific and rational than drug treatment after disease occurrence. The development and utilization has broad market prospects. According to the research and development status of Chinese plant resources, the development and utilization of medicine-food dual-use plants is facing challenges. Evaluation and selection of efficacy in research and development, study of functional substance basis and mechanism of action, functional product positioning and composition design, and plant resource germplasm evaluation, planting process management, post-harvest storage, etc. involved in functional food quality control process. A series of key technical issues, such as intensive processing and comprehensive utilization of resources, require efforts to carry out research in order to provide certain theoretical guidance and practice for the development and utilization of dual-use plant resources in China.
    2  Research and application of Lycii Fructus in medicinal field
    Jin Yang Yu-qing Wei Jian-Bao Ding Yan-long Li Jian-long Ma Jian-li Liu
    2018, 10(4):339-352. DOI: 10.1016/j.chmed.2018.08.006
    [Abstract](1019) [HTML](0) [PDF 0.00 Byte](7)
    Abstract:
    Lycii Fructus (Lycium barbarum, Gouqizi in Chinese name) is one of the most popular Chinese material medica and a common ingredient in tonic food. This fruit has been paid rapidly growing attention for its nutrient value and noticeable pharmacological properties. The present paper focuses on the legal resource of Gouqizi and reviews the main research in medicinal field including botanical identification, ethnopharmacological functions, phytochemistry, pharmacological effects, clinic usages, and safety issues. In addition, some issues needed address will be also discussed. We strongly believe that further investigation will deepen our knowledge of Gouqizi and promote the industrial development in the world.
    3  Taraxacum: Phytochemistry and health benefits
    Chun Hu
    2018, 10(4):353-361. DOI: 10.1016/j.chmed.2018.08.003
    [Abstract](1565) [HTML](0) [PDF 0.00 Byte](16)
    Abstract:
    Taraxaci Herba (Taraxacum mongolicum and other species) has been used in traditional Chinese medicine and dietary application for a long history in China, and Taraxacum officinale has been applied in medicinal and food use in other regions and cultures around the globe. In this review, the phytochemical constituents of dandelion (particularly from T. mongolicum and T. officinale) were summarized. Recent published health benefits of dandelion, such as anti-oxidant activity, anti-inflammatory activity, blood sugar and lipids regulation and hepatoprotective activity, as well as its safety data were highlighted. The limited human clinical study and pharmacokinetics information lead to the thought that well-designed human clinical study should be the focus and opportunity for the future research area to truly understand efficacy function and health benefit of dandelion for its application in medicine and health food area.
    4  Research and application progress of Gardenia jasminoides
    Fei Yin Jian-hui Liu
    2018, 10(4):362-370. DOI: 10.1016/j.chmed.2018.09.001
    [Abstract](594) [HTML](0) [PDF 0.00 Byte](7)
    Abstract:
    Fruits of Gardenia jasminoides (Zhizi in Chinese) have been used as dietary supplement and traditional Chinese medicine for thousands of years. Recent studies on Gardenia jasminoides reveal that the extracts or the active components play an essential role in oxidative stress, inflammation, hepatoprotection, neuroprotection, anti-diabetes, antitumor and so on. Here, we reviewed G. jasminoides in the aspects of botanical identity, chemical ingredients, pharmacology and pharmacokinetics, safety and toxicity evaluation, clinical application, etc.
    5  Values, properties and utility of different parts of Moringa oleifera: an overview
    Yang Liu Xiao-yue Wang Xue-min Wei Zi-tong Gao Jian-ping Han
    2018, 10(4):371-378. DOI: 10.1016/j.chmed.2018.09.002
    [Abstract](547) [HTML](0) [PDF 0.00 Byte](4)
    Abstract:
    Moringa oleifera is one of the most widespread species belonging to family Moringaceae. Almost each part of this tree has enormous properties in nutrition, medicine, or other industrial purposes. This review mainly introduces nutritional ingredients, bioactive compounds, applications and potentials of this tree, including leaves, seeds, pods, flowers, root barks and gum, in order to bring convenience to industries and researchers all over the world focusing on each part of this amazing plant. Moreover, the review also analyzes the current application situation of M. oleifera in China. After summarizing the recent researches of M. oleifera, we conclude that most current researches focus on the leaves and seeds of this plant, and the most concerned property of M. oleifera is the antioxidant ability. However, the molecular mechanisms under its multiple properties need further studies.
    6  Pharmacognostical study of Cynanchum stauntonii and Cynanchum glaucescens, Botanical Sources of TCM Baiqian
    Sutcharitchan Chayanis Li-na Wang Hang Zhang Xiao-die Li Ya-jun Cui Shen Ji
    2018, 10(4):379-387. DOI: 10.1016/j.chmed.2018.08.001
    [Abstract](784) [HTML](0) [PDF 0.00 Byte](4)
    Abstract:
    Objective Cynanchum stauntonii and Cynanchum glaucescens are botanical species of Baiqian (Cynanchi Stauntonii Rhizoma et Radix) in Chinese Pharmacopoeia, in which, however, there are no microscopic identification. So we provided the morphological and microscopic identification of the crude drug for updating Chinese Pharmacopoeia. Methods Twelve batches of C. stauntonii and three batches of C. glaucescens and their crude drugs were taxonomically, morphologically, and microscopically examined. Results Taxonomically, C. stauntonii had narrowly lanceolate leaves with acuminate apex and 5 mm long petiole; Whereas C. glaucescens was oblong-lanceolate or oblong with rounded or acute apex in leaves, and had very short or no petiole. Morphologically, rhizomes of C. stauntonii and C. glaucescens both had hollow pith, but the hollow pith occupied about a half of the rhizome’s diameter in C. stauntonii, whereas only a very small proportion of the overall diameter in C. glaucescens. Moreover, microscopic observation showed the difference in the proportion of xylem and in rhizome transverse-sections of the two species along with the difference in the size of the pith. Finally, laticifers and rhizome epidermal secretory cells were present in the powders of C. stauntonii, but absent from C. glaucescens. Conclusion Based on observation of morphological and microscopic characteristics, the two species can be distinguished by the size of the pith, proportion of xylem of rhizomes, and crude drug powder characters such as laticifers and secretory cells.
    7  Quality assessment of crude and processed Leonuri Fructus by chemical and color analysis combined with chemometric method
    Dong-hui Yan Xin-dan Liu Xian-mei Deng Bin Zhao Xing-yang Xue Shu-mei Wang Ying Zhang Jiang Meng
    2018, 10(4):388-395. DOI: 10.1016/j.chmed.2018.07.006
    [Abstract](948) [HTML](0) [PDF 0.00 Byte](9)
    Abstract:
    Objective: Crude Leonuri Fructus (CLF), the fruits of the Leonurus japonicus, and processed Leonuri Fructus (PLF) by stir-baking as the important Chinese herbal medicines, have been used in China and other Asian countries for thousands of years. The objective of this research is to reveal the difference between CLF and PLF. Methods: The sensory technologies of the colorimetry, sensitive and validated HPLC-ELSD and GC combined with flame ionization detector (GC-FID) were employed to discriminate CLF and its processed product PLF. The color parameters of the samples were determined by colorimetric instrument CR-410. Moreover, the content of stachydrine and six fatty acids were determined by HPLC and GC. Subsequently, analysis of variance (ANOVA), principal components analysis (PCA), hierarchical cluster analysis (HCA), and Kendall’s correlation test were performed for data analysis. Results: The CLF and PLF were divided into two categories by PCA and HCA in terms of their component content and color. The results distinctly demonstrated significant changes in color and the content of indicative components between CLF and PLF. Conclusion: The study revealed that HPLC, GC, and colorimetric method in combination with chemometric method could be used as comprehensive quality evaluation for CLF and PLF.
    8  Multivariate modeling to evaluate effect of fabricating process on product profile of high shear granulation
    Zheng Yan Ying-ying Gong Liang-shan Ming Yi-hui Xie
    2018, 10(4):396-404. DOI: 10.1016/j.chmed.2018.07.005
    [Abstract](415) [HTML](0) [PDF 0.00 Byte](3)
    Abstract:
    Objective: This study aims to investigate the feasibility of high shear granulation (HSG) for agglomeration of Crataegi Folium extract (Chinese edible herbal), and explore the effect of process variables on granule critical quality attributes (CQAs) by three different models, i.e., response surface methodology (RSM), multilayer perceptron neural networks (MLP), and partial least squares method (PLS). Method: RSM, MLP, and PLS complementary to design of experiment (DoE) were utilized to investigate the influence of granulation time, impeller speed, and binder amount on the products. Crataegi Folium extract was employed as granulation powder. RSM was further carried out to develop design space of HSG. Results: The results indicated that RSM, MLP, and PLS modeling techniques enhanced the understanding and controlling of granules produced via HSG. The granule CQAs were mainly influenced by granulation time, impeller speed, and binder amount. Overlay plots of the RSM indicated that design space for the operating ranges of impeller speed and binder amount at high levels of granulation time was the smallest. Conclusion: The study showed that these models were useful to characterize the granulation process, and was particularly important to understand the process.
    9  Mechanism of Chinese herbal formula QHF against breast cancer MCF-7 cells invasion and migration
    Wei Hu Dan Liu Liang-bo Jiao Wen Zhang Xiang-yang Hu Shen-tong Gan Xin-yi Wang Tao Chen
    2018, 10(4):405-410. DOI: 10.1016/j.chmed.2018.08.004
    [Abstract](560) [HTML](0) [PDF 0.00 Byte](8)
    Abstract:
    Objective: To investigate the effects of Chinese herbal formula Qinghuofu (QHF) on the migration and invasion of breast cancer MCF-7 cells and its possible molecular mechanisms, thereby providing a theoretical basis to find effective anti-cancer medicine and therapeutic targets for the treatment of anti-migration and anti-invasion of breast cancer. Methods: Breast cancer MCF-7 cells were treated with different QHF and other different reagents, CCK8 assay was used to detect the influence of the reagents on the proliferation of MCF-7 cells; Scrape migration and Transwell assay were used to quantitatively determine the migration and invasion effects of QHF and HGF on the MCF-7 cells. Subsequently, the c-Met inhibitor and its downstream ERK and PI3K inhibitors were used to investigate the relationship between the migration and invasion of MCF-7 cells, as well as its downstream MAPK/ERK and PI3K/Akt signaling pathways. The expression levels of HGF, c-Met, ERK, p-Akt, p-c-Met, p-ERK, p-Akt, MMP2, MMP9, and VEGF in breast cancer MCF-7 cells treated with QHF and other reagents were also examined. Results: The result indicated that formula QHF not only significantly inhibited the proliferation of MCF-7 cells, but also significantly suppressed the effects of (hepatocyte growth factor) HGF (40 ng/mL) on the proliferation and movement of MCF-7 cells, reducing the ability of the cells to invade and migrate. Western blot analysis indicated that QHF and c-Met inhibitor significantly decreased the expression of p-c-Met, p-ERK1, p-ERK2, p-Akt, MMP-2, MMP-9, and VEGF, while HGF could significantly increase the expression of p-c-Met in MCF-7 cells; c-Met downstream ERK and PI3K inhibitors could also significantly decreased the expression of MMP-2, MMP-9, and VEGF in MCF-7 cells; But the difference among c-Met, PI3K, ERK, and QHF group were not statistically significant. Conclusion: QHF can prevent the proliferation, migration, and invasion of MCF-7 cells by inhibiting the HGF/c-Met and its downstream PI3K/Akt and MAPK/ERK signaling pathways; Thereby down-regulating the expresssion of HGF, p-Met, p-ERK1, p-ERK2, p-Akt, MMP-2, MMP-9, and VEGF.
    10  Antifibrosis effects of Shenge Yangfei Capsules on bleomycin-induced pulmonary fibrosis in rats
    Hui Jia Zuo-jun Wang Qiong Wu Zi-ru Xu Hong-da Ma Qing-chun Zhao
    2018, 10(4):411-415. DOI: 10.1016/j.chmed.2018.07.007
    [Abstract](836) [HTML](0) [PDF 0.00 Byte](5)
    Abstract:
    Objective: The present study, for the first time, was designed to examine the antifibrosis effects of Shenge Yangfei Capsules (SGYF) on bleomycin-induced pulmonary injury and fibrosis in rats. Methods: Animals were divided into six groups randomly: saline control group, bleomycin group, and SGYC (70, 420 or 850 mg/kg/d, ig) group, and positive control group. Animals were sacrificed on day of 7th, 14th, and 28th post bleomycin administration, and lungs were removed respectively. For histological evaluation of lungs injury and fibrosis, lung specimens were stained with hematoxylin and eosin (HE) and Masson trichrome. Body weight and lung index from various groups were measured, and the concentrations of monoamine (MAO) and transforming growth factor-β1(TGF-β1) were also detected in lung homogenates. Results: SGYC attenuated the bleomycin-induced weight loss and the lung index. Histologica levidence showed the ability of SGYC to decrease bleomycin-induced lung fibrosis and consolidation. SGYC reduced the MAO and TGF-β1 activityin lung tissues. Conclusions: These findings suggest that SGYC may be a promising candidate to prevent bleomycin-induced lung damage or other interstitial lesions.
    11  Optimization of enzyme assisted extraction of polysaccharides from pomegranate peel by response surface methodology and their antioxidant potential
    Yun Li Cai-ping Zhu Xi-chuan Zhai Yang Zhang Zhen Duan Jing-ru Sun
    2018, 10(4):416-423. DOI: 10.1016/j.chmed.2018.08.007
    [Abstract](566) [HTML](0) [PDF 0.00 Byte](6)
    Abstract:
    Objective To optimize the enzymatic extraction conditions of polysaccharides from pomegranate peel using response surface methodology (RSM), and invest the anti-oxidant activity of pomegranate peel polysaccharide in vitro for seeking novel biological components used as pharmaceutical products and functional foods. Methods Effects of enzymolysis time, ratio of water to raw material, and the dosage of cellulase on the extraction yield of pomegranate peel polysaccharide were fully examined by Box-Behnken design of RSM. DPPH radical scavenging activity, hydroxyl radicals scavenging activity, superoxide anion radical scavenging activity, and reducing power assay were measured by microplate reader. Results The optimal extraction conditions were as follows: enzymolysis temperature 55 ?C, pH 5.0, enzymolysis time 88 min, ratio of water to raw material 22:1 mL/g, and dosage of cellulose 0.93%. Under the optimal extraction conditions, the yield of pomegranate peel polysaccharide was (22.31 ± 0.07)%, which was well matched with the predicted value 22.35% of the Box-Behnken design model. Also, pomegranate peel polysaccharide demonstrated appreciable anti-oxidant potential on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging and reducing powerin vitro. Conclusions An optimized enzymolysis assisted method was proposed for the extraction of pomegranate peel polysaccharide, which can be used as a good anti-oxidant.
    12  Intervention effects of Compound Houttuyniae Herba to diabetic renal damage based on SOCS-JAK/STAT negative feedback regulation
    Yun Fang Sai-cong Shao Hai-ying Wang
    2018, 10(4):424-430. DOI: 10.1016/j.chmed.2018.08.005
    [Abstract](868) [HTML](0) [PDF 0.00 Byte](6)
    Abstract:
    Objective To research the protective effects of different extracts from Compound Houttuyniae Herba (CHH) and its mechanism through JAK/STAT-SOCS-1 signaling pathway. Methods The normal group comprised db/m mice (n = 8). db/db mice were randomly divided into seven groups with eight mice in each group according to the applied treatment method: model group, metformin hydrochloride (MH) group, AG490 group, water extract (WE) group, ethanol extract (EE) group, volatile oil (VO) group, and mixture (MG) group (mixture of above three extract) of CHH. After 8 weeks, the general status, biochemical indicators, and renal histological changes in the mice were evaluated, the total RNA and protein were collected and RT-PCR method was used to examine the effect on mRNA expression of JAK2, STAT3, SOCS1, and Western blot method was used to detect the protein expression of JAK2, P-JAK2, STAT3, P-STAT3, SOCS1, c-fos, and c-jun. Immunofluorescence was used to observe the protein expression of c-fos and c-jun in kidney tissue. Results Compared with normal group, the serum level of TGF-β1, FN of EE, VO, and MG groups were decreased (P < 0.05). Renal function was also improved but not significantly. Also, the renal histology was improved especially in the mixture group. The protein expression of JAK2, STAT3, P-JAK2, P-STAT3, and the genetic expression of JAK2 and STAT3 in kidney tissue were significantly increased in the model group (P < 0.05). Compared with the model group, the expression of P-JAK2 and P-STAT3 was down-regulated in treatment groups; The expression of SOCS-1 of VO and mixture groups were elevated (P < 0.05). Conclusion CHH has beneficial effects on diabetic renal injury, which may protect and improve the kidney function and reduce urinary protein, maintain the integrity of the kidney structure and function.
    13  Potential mechanism of cholagogic effect of Gardenia jasminoides Zhizi: Reduction of hepatic bile acids level by increasing urinary excretion via up-regulating renal Mrp2 in normal rats
    Fan Zhang Yu-hui Wei Ying-ting Duan Yan-shu Zhao Li-li Xi Zhi Rao Jian-ping Zhang Guo-qiang Zhang Xi-nan Wu
    2018, 10(4):431-436. DOI: 10.1016/j.chmed.2018.08.008
    [Abstract](800) [HTML](0) [PDF 0.00 Byte](2)
    Abstract:
    Objectives Gardenia Jasminoides (Zhizi in Chinese), belonging to Rubiaceae family, has been traditionally used to treat cholestasis and jaundice for centuries in Asia. In the theory of traditional Chinese medicines, Zhizi could dispel dampness and heat via the urine to execute its choleretic effects. However, the potential molecular mechanism is still poorly clarified. Here, we investigated the effect of different dose of Zhizi aqueous extracts powder on urinary excretion of bile acids (BAs), and explored the potential mechanism via renal BAs efflux transporters Mrp2 and Mrp4 in normal rats. Methods Male Wistar rats were orally administrated with 0.3 or 0.9 g/kg/d dose of Zhizi aqueous extract powder for 2 weeks, then body weight, serum aminotransferase, total BAs concentrations in liver, bile, serum, kidney, and urine, 1 h bile flow, 12-h urinary volume, biliary, and urinary excretion amount of total BAs as well as protein expression of major renal BAs efflux transporter Mrp2 and Mrp4, were all evaluated. Results Zhizi especially the high dose of Zhizi aqueous extract powder reduced hepatic total BAs concentration. Additionally, bile flow and biliary excretion had no significant difference, but the remarkable increased urinary excretion of BAs and 2 ? 3 folds up-regulated renal Mrp2 expression were observed after administrated with Zhizi as compared with the control group. Conclusion The findings indicated that Zhizi reduced hepatic total BAs level by increasing urinary excretion rather than the biliary excretion of BAs, which in turn ascribed to elevated protein expression of Mrp2 at apical membrane of renal tubular epithelial cells.
    14  Xanthotoxin induces apoptosis in SGC-7901 cells through death receptor pathway
    Xiu-juan Zhang Cang Wei Li-juan He Jian An
    2018, 10(4):437-444. DOI: 10.1016/j.chmed.2018.08.009
    [Abstract](907) [HTML](0) [PDF 0.00 Byte](10)
    Abstract:
    Objective To investigate the effects of xanthotoxin from Apiaceae medicinal plants on cell proliferation and apoptosis, and explore its mechanism of action against human gastric carcinoma SGC-7901 cells in vitro. Methods SGC-7901, HepG-2, MCF-7, and A549 cells were treated with different concentrations of xanthotoxin (10, 20, 60, 80, 100, 120, 140, and 160 μg/mL) for 48 h, and the cell viability (IC50) was determined by MTT assay; Xanthotoxin-induced apoptosis in cells was observed by using Hoechst 33258 Staining Kit and Annexin V-FITC Apoptosis Detection Kit; Flow cytometry was used to detect apoptosis related proteins of Fas/FasL, Bid, and DR5/TRAIL proteins in human gastric carcinoma SGC-7901 cells after being treated by xanthotoxin; The influence of xanthotoxin on Caspase-8 protein expression in the cells was determined by Flouormetric Assay Kit. Results Xanthotoxin obviously inhibited SGC-7901, HepG-2, MCF-7, and A549 cells proliferation, and its inhibition was in a concentration-dependened manner; Flow cytometry results showed that in a certain concentration range, xanthotoxin can increase the expression levels of Fas/FasL and DR5/TRAIL proteins in a concentration-dependence manner. The content of bid protein in cells was increased, and it showed concentration-dependence. Conclusion Xanthotoxin may induce SGC-7901 cells apoptosis in a certain concentration range through the Fas/FasL protein mediated death receptor pathway, or by DR5/TRAIL mediated death receptor pathway, and increase the expression level of death receptor protein, activation Caspase-8, activating downstream effect factor, inducing cell apoptosis, or activate Caspase-8 cutting activate protein Bid, and then enter the mitochondrial pathway, induction of apoptosis.
    15  Effect of cinnamaldehyde on 1, 3-β-d-glucans in cell wall of Candida albicans in immunosuppressed BALB/c mice
    Jie-hua Deng Gang-sheng Wang Ji-hong Li Xiao-lu Wang
    2018, 10(4):445-450. DOI: 10.1016/j.chmed.2018.08.002
    [Abstract](874) [HTML](0) [PDF 0.00 Byte](8)
    Abstract:
    Objective: Invasive pulmonary candidiasis is a disease with high incidence, difficult treatment, poor prognosis, and high mortality. The present study analyzed the influence of cinnamaldehyde on 1,3-β-D-glucans in the cell wall of Candida albicans in order to provide a theoretical basis for the research of antifungal drugs. Methods: An immunosuppressed BALB/c mouse model with invasive pulmonary candidiasis was established by nasal perfusion of 50 μL of C. albicans suspension (107 cfu/mL). 1,3-β-D-glucans examination and electron microscopy were carried out. Fluconazole was used as the control. Results: Cinnamaldehyde was administered at a dose of 240 mg/kg/d for 14 consecutive days, and the measured value of 1,3-β-D-glucans was (1160.62 ± 89.65) pg/mL, whereas that of fluconazole was (4285.87 ± 215.62) pg/mL. The difference between the two groups was statistically significant (P < 0.05). Electron microscopy observation indicated that the 2?3 layers outside the cell wall of C. albicans (1,3-β-D-glucans layer) were rough, deformed, and incomplete, although the cell membrane was clear and intact. Conclusion: Cinnamaldehyde demonstrated special efficacy on 1,3-β-D-glucans in the cell wall of C. albicans.

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