Volume 5,Issue 1,2013 Table of Contents

1 Chemical Constituents in Charred Sanguisorbae Radix

SUN Li-li , ZHONG Ying , XIA Hong-min , ZHOU Qian , LV Jia

2013, 5(1):1-4. DOI: 10.7501/j.issn.1674-6384.2013.01.001

[Abstract](1115) [HTML](0) [PDF 306.32 K](2423)

Abstract:
Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)- en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1, and Y2, respectively.

2 A New Phenylpropanol Glycoside and Its Five Known Analogues from Boschniakia rossica

WU Chun-hua , WANG Tao , YAMAGUCHI Yoshihiro , CHEN Yue , HAN Li-feng , ZHANG Yi

2013, 5(1):5-8. DOI: 10.7501/j.issn.1674-6384.2013.01.002

[Abstract](1044) [HTML](0) [PDF 213.78 K](3018)

Abstract:
Objective To study the constituents in the whole plant of Boschniakia rossica. Methods The constituents were separated and purified with chromatographic methods. Their structures were elucidated by spectroscopic methods (1D, 2D NMR, UV, IR, and HRESI-TOF-MS) and chemical analyses. Results One new phenylpropanol glycoside (1) and its five known analogues were obtained from B. rossica. They were identified as trans-p-coumaryl- (2′-O-β-D-glucopyranosyl)-β-D-glucopyranoside (1), salidroside I (2), rossicasin A (3), trans-p-coumaryl alcohol 1-O-β-glucopyranosyl(1→4)-α-rhamnopyranosyl(1→3)-β-glucopyranoside (4), salidroside (5), and acetoside (6). Conclusion Among the known compounds, compound 2 is firstly isolated from the plants in genus Boschniakia C. A. Mey. ex Bongard. Meanwhile, the 13C-NMR data of 9 and 4′ positions in compound 4 are corrected.

3 Review of Rhubarbs: Chemistry and Pharmacology

ZHENG Qing-xia , WU Hai-feng , GUO Jian , NAN Hai-jiang , CHEN Shi-lin , YANG Jun-shan , XU Xu-dong

2013, 5(1):9-32. DOI: 10.7501/j.issn.1674-6384.2013.01.003

[Abstract](2857) [HTML](0) [PDF 583.26 K](10971)

Abstract:
Rhubarb is a perennial herb belonging to the genus Rheum L. (Polygonaceae). Rhei Radix et Rhizoma (rhubarb roots and rhizomes) is one of the most popular Chinese materia medica and has been widely used for strong laxative function. About 200 compounds with six different types of skeletons (anthraquinone, anthrone, stilbene, flavonoids, acylglucoside, and pyrone) have so far been isolated from eighteen species of the genus Rheum L. These constituents showed extensive pharmacological activities including cathartic, diuretic, anticancer, hepatoprotective, anti-inflammatory, and analgesic effects, as well as toxicological effects. Chemical fingerprint, LC-MS, and other analytical techniques have been used for the quality control of rhubarb. This comprehensive review summarizes the researches into the isolation, pharmacological activities, and phytochemical analysis reported since investigations began in the late 1940s. In addition, pharmacokinetic studies and clinical application of rhubarb are also discussed in present paper.

4 Phytochemistry, Pharmacology, Toxicology, and Structure-Cytotoxicity Relationship of Paridis Rhizome Saponin

MAN Shu-li , WANG Yan-li , LI Yuan-yuan , GAO Wen-yuan , HUANG Xian-xiao , MA Chao-yi

2013, 5(1):33-46. DOI: 10.7501/j.issn.1674-6384.2013.01.004

[Abstract](1485) [HTML](0) [PDF 191.54 K](3834)

Abstract:
The rhizomes of Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis are used as traditional herbal medicines in many parts of China. The Paridis Rhizome saponin (PRS), as the active ingredient, has played an important role in hemostasis, antibacterial action, and inflammation counteraction, bearing some analogy to Gongxuening and Yunnanbaiyao in efficacy. Modern pharmacological experiments have proved that PRS possesses two main sapogenins: diosgenin and pennogenin, which could provide a lot of clinical treatment effects (anti-oxidation, anti-inflammation, anti-apoptosis, anti-metastasis, and immunostimulant, etc.). In the past, several main steroid saponins have been studied in a number of randomized controlled trials for their effects and mechanisms mainly on antitumor performance. The extensive results have demonstrated that PRS was an effective group of active components to antitumor clinical trials. In this article, we reviewed the reported phytochemical, pharmacological, and toxicological properties of PRS and compared the structure-cytotoxicity relationship of PRS in antitumor effects.

5 Alleviation of PEGylated Puerarin on Erythrocyte Hemolysis Induced by Puerarin in Glucose-6-phosphate Dehydrogenase- deficient Rats

LIU Xin-yi , LI Jian-rong , WANG Nai-jie , ZHANG Guang-ping , DU Feng , YE Zu-guang , XIANG Da-xiong

2013, 5(1):47-52. DOI: 10.7501/j.issn.1674-6384.2013.01.005

[Abstract](1544) [HTML](0) [PDF 225.02 K](3079)

Abstract:
Objective To explore and analyze the reducing hemolytic effects of PEGylated puerarin (PEG-PUE) on erythrocytes induced by PUE in glucose-6-phosphate dehydrogenase (G6PD)-deficient rats. Methods The rat model with G6PD-deficiency was established via sc injecting 1% acetylphenyl-hydrazine. Then the G6PD-deficient erythrocyte suspension obtained from this rat model was used to evaluate the hemolytic effects of PUE and the reducing hemolytic effects of PEG-PUE via hemolytic activity and erythrocyte osmotic fragility assay. Results It was found that PUE could cause a serious hemolysis to the erythrocyte suspension with the increase of drug concentration and the prolongation of drug incubation time, the hemolytic rate of PUE was up to 40%, while the addition of PEG-PUE to the erythrocyte suspension revealed no significant hemolysis. Additionally, the result of erythrocyte osmotic fragility indicated that PEG-PUE exerted a slight effect on the erythrocyte membranes, and the NaCl concentration that induced 50% hemolysis (32 mmol/L) was about one-third PUE. Conclusion These results demonstrate that PEG-PUE could play a significant role in reducing the side effect of hemolysis induced by PUE. The low hemolytic activity of PEG-PUE makes it a favorable candidate for in vivo tests and PEG-PUE could also provide the useful insight for the further formulation development as an innovative drug.

6 Anti-hepatitis B Virus Activity of 8-epi-Kingiside in Jasminum officinale var. grandiflorum

ZHAO Gui-qin , YIN Zhi-feng , LIU Li-yan , MAO Xiao-xia , SU Zhan-hui

2013, 5(1):53-57. DOI: 10.7501/j.issn.1674-6384.2013.01.006

[Abstract](1285) [HTML](0) [PDF 187.08 K](2650)

Abstract:
Objective To evaluate the effect of 8-epi-kingiside (8-Epik) derived from the buds of Jasminum officinale var. grandiflorum (JOG) on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo. Methods The concentration of extracellular hepatitis B e antigen and hepatitis B surface antigen (HBsAg) in cell culture medium was determined by ELISA, respectively. The anti-HBV effects of 8-Epik were also demonstrated in the model of DHBV. 8-Epik was ip given (20, 40, and 80 mg/kg, twice daily) to the DHBV-infected ducklings for 10 d. The isotonic saline liquid diet was ip given as negative control and Lamivudine (50 mg/kg, twice daily) was given as positive control. DHBV DNA was measured at days 0 (T0), 5 (T5), 10 (T10), and day 3 after cessation of treatment (P3) by dot blotting. Results 8-Epik effectively blocked HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner [IC50 = (19.4 ± 1.04) μg/mL]. 8-Epik (40 or 80 mg/kg, ip, twice daily) also reduced viremia in DHBV-infected ducks. Conclusion Therefore, 8-Epik is warranted as a potential therapeutic agent for HBV infection.

7 Effect of Qinglongyi Polysaccharides on Complex Mobility of Erythrocytes in S180 Mice

JI Chen-feng , YUE Lei , JI Yu-bin

2013, 5(1):58-61. DOI: 10.7501/j.issn.1674-6384.2013.01.007

[Abstract](856) [HTML](0) [PDF 171.41 K](2132)

Abstract:
Objective To study the effect of Qinglongyi polysaccharides (QP) in the exocarp of Juglans mandshurica on the complex mobility of erythrocytes in S180 mice. Methods Erythrocytes were collected and prepared into suspensions, and the complex mobility of cells was measured using high performance capillary electrophoresis (HPCE). Optimized experimental conditions were as follows: 50 cm × 75 μm capillary, buffer for electrophoresis; phosphate solution containing hydroxypropylmethyl cellulose (0.1 mol/L, pH 7.4), injection pressure 3.448 kPa, injection time 10 s, separation voltage 20 kV, and column temperature 25 ℃. Results The migration time of erythrocytes in S180 mice was longer than that in normal mice, which was 18.09 min for the model group and 12.11 min for the control group, and the complex mobility of erythrocytes in S180 mice was lower than that in normal mice, which was 0.92 × 10?4 cm2/(V?s) for the model group and 1.38 × 10?4 cm2/(V?s) for the control group. It was also found that S180 mice treated by QP could shorten the migration time and increase the complex mobility of erythrocytes. Conclusion QP could improve the complex mobility of erythrocytes in S180 mice, and HPCE could be used as a powerful tool for determining the physiological state and functions of erythrocytes.

8 Protective Effects of Lentinan against T Lymphocytes Injury in Mice under Chronic Radiation Stress

WANG Yong , LI Ming-chun , FU Qing-jie

2013, 5(1):62-66. DOI: 10.7501/j.issn.1674-6384.2013.01.008

[Abstract](885) [HTML](0) [PDF 282.66 K](2241)

Abstract:
Objective To study the effects of lentinan (LTN) on mice exposed to chronic radiation. Methods Animals were divided into three groups (n = 10), they were animals exposed to radiation (Rad), normal control animals (Ctr), and irradiated animals treated with LTN (Rad + LTN). Animal model of chronic radiation stress injury was induced by irradiating mice with 60Co γ-ray for 6 weeks from Monday to Friday consecutively. Before radiation, the mice in Rad + LTN group were ip injected with 0.5 mL LTN (0.01 mg/mL), whereas mice in other groups were injected with 0.9% physiological saline. The effects of LTN treatment on irradiated mice were examined by histological analysis on the spleen. The cell numbers and viability of T lymphocytes, which were isolated from the spleen, were determined by Trypan blue staining. Nitric oxide (NO) production and interleukin-2 (IL-2) secretion in T lymphocytes were also measured. Results Chronic radiation significantly reduced the body weights and the spleen and thymus indexes, associated with reduced T lymphocytes viability and functions, and elevated NO production. Treatment with LTN significantly normalized the elevated NO production, and attenuated the negative outcomes resulting from radiation mentioned above. Conclusion The results suggest that radioprotective effect of LTN may be contributed by improved T lymphocytes viability and functions via regulating the NO and IL-2 production in T lymphocytes.

9 Aqueous Two-phase Systems with Ultrasonic Extraction Used for Extracting Phenolic Compounds from Inonotus obliquus

ZHAO Yan-xia , LIU Yu-bing , LIU Feng , ZHENG Wei-fa

2013, 5(1):67-72. DOI: 10.7501/j.issn.1674-6384.2013.01.009

[Abstract](1378) [HTML](0) [PDF 169.45 K](2913)

Abstract:
Objective To optimize the extracting technology of assessing the maximum yield of phenolic compounds (PC) from Inonotus obliquus by single factor experiments and orthogonal array design methods through aqueous two-phase systems combined with ultrasonic extraction. Methods The range of the independent variables, namely levels of acetone and ammonium sulfate, and ultrasonic time were identified by a first set of single factor experiments. The actual values of the independent variables coded at four levels and three factors were selected based on the results of the single factor experiments. Subsequently, the levels of acetone and ammonium sulfate, and ultrasonic time were optimized using the orthogonal array method. Results The optimum conditions for the extraction of PC were found to use 7.0 mL acetone, 5.5 mg ammonium sulfate, with ultrasonic time for 5 min. Under these optimized conditions, the experimental maximum yield of PC was 37.8 mg/g, much higher than that of the traditional ultrasonic extraction (UE, 29.0 mg/g). And the PC obtained by this method had stronger anti-oxidative activities than those by traditional UE method. Conclusion These results indicate the suitability of the models developed and the success in optimizing the extraction conditions. This is an economical and efficient method for extracting polyphenols from I. obliquus.

10 GC-MS Analysis on Volatile Oil from Branches of Picrasma quassioides

HUANG Lin-fang , WANG Zeng-hui , CHEN Shi-lin

2013, 5(1):73-76. DOI: 10.7501/j.issn.1674-6384.2013.01.010

[Abstract](1348) [HTML](0) [PDF 117.34 K](2588)

Abstract:
Objective To analyze the volatile oil from the branches of Picrasma quassioides. Methods Volatile oil was extracted from the branches of P. quassioides by steam distillation. GC-MS method was used to analyze the components. Results A total of 49 compounds were isolated, and 46 compounds were successfully identified, which represented over 98% of the total oil composition. The major components of the volatile oil in the branches of P. quassioides included caryophyllene (12.83%), 12-oxabicyclo[9.1.0]dodeca-3,7-diene,1,5,5,8-tetramethyl-[1R- (1R*,3E,7E,11-R*)] (12.29%), 1-hexanol (9.96%), naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7- (1-methyletheyl)-[1S-(1a,7a,8aa)] (7.32%), aromadendrene oxide-(2) (6.69%), and α-caryophyllene (3.88%). Conclusion The major components in volatile oil are terpenoids, hydroxy compounds, and other acyclic alkane compounds.

Volume 5,Issue 1,2013 Table of Contents

Current Issue

Volume

Issue

Most Read

Most Cited

Most Downloaded

WeChat

Mobile website