Antibacterial and antibiofilm activity of novel nanofibers bandage formulated with Juniperus communis essential oil targeting antibiotic resistant bacterial strains
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摘要:
目的：抗菌药物耐药性（AMR）的急剧上升威胁着当前抗生素的治疗手段。这一全球性的公共卫生威胁促使人们重新开始寻找植物来源的抗菌疗法。欧洲刺柏（Juniperus communis L.）在民间医学中已有数百年使用历史。本研究旨在评估以从欧洲刺柏中提取的精油制成的聚己内酯（PCL）纳米纤维敷料，对临床常见且易引起医院感染的ESKAPE细菌病原体（包括大肠杆菌、粪肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和阴沟肠杆菌）的抗菌及抗生物膜活性。 方法：采用细菌生长曲线和杀菌试验，对敏感和耐药的临床细菌菌株（统称为ESKAPE病原体）进行检测。通过96孔板分别测试欧洲刺柏精油（JEO）对耐甲氧西林金黄色葡萄球菌（MRSA）和大肠杆菌的抗生物膜活性，将含细菌的96孔板过夜培养以促进生物膜形成，随后用结晶紫染色。采用纸片扩散法测试了不同浓度（2%、4%、6%和8%）JEO配制的PCL基纳米纤维膜的抗菌活性。 结果：JEO稀释液（50、25和12.5 μL）对所测试菌株表现出剂量依赖性的抗菌活性，24小时后显著抑制了细菌生长。JEO还对耐抗生素的MRSA和大肠杆菌菌株表现出抗生物膜活性。此外，含8% JEO的PCL纳米纤维膜在琼脂平板上显示出较大的抑菌圈，有效抑制了MRSA的生长。 结论：JEO对ESKAPE病原体具有抗菌活性。将JEO负载于PCL纳米纤维敷料中，有望用于伤口敷料，治疗手术部位感染、烧伤或皮肤溃疡。
Abstract:
Objective: The alarming increase in antimicrobial resistance (AMR) threatens current armament of antibiotics. This global threat to public health revived the search for plant-derived antimicrobial therapeutics.Juniperus communis L. has been used in folk medicine for centuries. This study aims to examine the antibacterial and antibiofilm activity of polycaprolactone (PCL) nanofiber bandages formulate with essential oil extracted from J. communis against clinically relevant ESKAPE (Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae,Acinetobacterbaumanii, Pseudomonas aeruginosa, and Enterobacter cloacae) bacterial pathogens commonly causing nosocomial infections. Methods: Bacterial growth curves and bactericidal assays were performed on antibiotic sensitive and resistant clinical bacterial strains collectively known as ESKAPE pathogens. Antibiofilm activity of Juniper essential oil (JEO) was tested against methicillin resistant S. aureus (MRSA) and E. coli individually in a 96-well plate, and the 96 well plate which contains the bacteria was incubated over night to allow biofilm formation and stained using crystal violet. The antibacterial activity of PCL-based nanofiber membrane formulated with various doses (2%, 4%, 6%, and 8%) of JEO was tested using disc diffusion method. Results: JEO dilutions (50, 25 and 12.5 lL) demonstrated a dose-dependent antibacterial activity against tested strains and significantly reduced growth after 24 h. JEO also exerted antibiofilm activity against MRSA and E. coli strains that are resistant to antibiotics. Additionally, PCL nanofiber membranes formulated with 8% JEO inhibited MRSA growth as demonstrated by large zone of inhibition on agar plate. Conclusion: JEO has antibacterial activity against ESKAPE pathogens. The potential application of JEO formulated in PCL nanofiber bandages is for wound dressing to treat surgical site infections, burns or skin ulcers.
关键词:
抗菌素耐药性;ESKAPE病原菌;精油;欧洲刺柏;纳米纤维绷带
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Project Supported:
This research is funded by Qatar Research, Development, and Innovation (QRDI) Council Grant (No. UREP30-151-3-048). Authors thank Dr Mathew Donadu, University of Sassari, Sassari, Italy for the kind gift of JEO. Authors thank Dr Layla Kamareddine at Qatar University for the kind gift of GFP-labelled E. coli strain. Authors acknowledge the Central Laboratory Unit at Qatar University for providing scanning electron microscopy images of the formulated PCL nanofibers.
Mohannad N. AbuHaweeleh a,, Ahmad Hamdan a, ,, Menatalla Metwally Said a, Maryam Hassiba a, Robin Augustine b, Anwarul Hassan c, Nahla O. Eltai d, Susu M. Zughaier a,?. Antibacterial and antibiofilm activity of novel nanofibers bandage formulated with Juniperus communis essential oil targeting antibiotic resistant bacterial strains[J]. Chinese Herbal Medicines (CHM),2026,18(3):758-766
