Objective: Yu-Ping-Feng-San (YPFS) has been found to significantly reduce exacerbation rate and the risk of a second exacerbation in patients with chronic obstructive pulmonary disease (COPD). However, the effect of YPFS on emphysema development and its exacerbation caused by influenza virus is still unclear. This study aims to investigate the protective effect of YPFS on the impaired lung function and increased inflammation that occurs during the development and exacerbation of emphysema. Methods: This study developed an emphysema mice model using porcine pancreatic elastase (PPE) and treated it with YPFS [1 950, 975, and 487.5 mg/(kg d)] once daily. On day 7 post-PPE challenge, peripheral leucocytes and inflammatory cells in lungs were analyzed, respectively. Pulmonary inflammatory mediators were determined. On day 28, invasive lung function and inflammatory mediators were measured. In addition, histopathological changes at both time points were assessed. We also developed an exacerbation of emphysema mouse model by intratracheally infected mice with influenza H1N1 virus on day 28. After infection, YPFS administration was discontinued, and the protective effect was determined by lung function, viral titer and cytokine levels in the lungs, and lung histopathological changes on day 5 postinfection. Results: The results demonstrated that 32 days of YPFS administration significantly improved lung function and reduced severity of emphysema in PPE-treated mice. It significantly reduced monocytes and neutrophils, while increased lymphocytes in peripheral blood. Additionally, YPFS inhibited the accumulation of alveolar macrophages and the expression of cytokines in mice treated with PPE. MMP-9, fibronectin, and VEGF, which are inflammatory mediators associated with airway remodeling, were also inhibited by YPFS. The results also demonstrated that YPFS prophylaxis prior to viral infection benefited mice treated with PPE and H1N1 as evidenced by decreased lung cytokine levels, lowered lung index, and improved lung histopathology. Conclusion: YPFS prevents the development of emphysema and its exacerbation induced by influenza virus in mice. Our finding provides scientific evidence for the prophylaxis application of YPFS in mitigating acute exacerbation of chronic obstructive pulmonary disease (AECOPD).
关键词:
肺气肿;急性加重;炎症;流感病毒;玉屏风散
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Project Supported:
This study was funded by National Administration of Traditional Chinese Medicine (No. ZYYCXTU-D-202203), the National Natural Science Foundation of China (No. 82374304, 81872765), Guangdong Academy of Sciences (No. 2020GDASYL-20200103046), Young top talent of science and Technology Innovation Department of Guangdong Province (No. 2021TQ060189), Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Disease (No. GHMJLRID-Z-202105), Zhongnanshan Medical Foundation of Guangdong Province (No. ZNSA-2020012, ZNSA-2020013), Open Project of State Key Laboratory of Respiratory Disease (No. SKLRD-OP-202215), and Guangdong Basic and Applied Basic Research Foundation (No. 2020B1515120045, 2020A1515110151).
Lingzhu Deng a, ,, Yaorong Chen a,, , Ruifeng Chen a, c, Shengle Qin a, Xiao Wu a, Shiyun Liang d, Tongmei Shi d, Xin Zhao e, Runfeng Li a, Zifeng Yang a, b,c, f. Yu-Ping-Feng-San mitigates development of emphysema and its exacerbation induced by influenza virus in mice[J]. Chinese Herbal Medicines (CHM),2026,18(2):294-302
