Anthraquinones of Cassiae Semen alleviate lipid accumulation in obesity by regulating brown adipose tissue and liver function
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摘要:
目的：决明子是一种广泛应用的传统中药，具有多种药理作用。本研究旨在探究决明子中蒽醌类化合物（AQS）对肥胖的潜在治疗效果及其分子机制。方法：研究采用高效液相色谱法（HPLC）测定了AQS的主要成分，随后利用网络药理学分析预测了AQS潜在的抗肥胖作用靶点。通过构建高脂高糖水饮食诱导的肥胖小鼠，观察其体重变化和全身脂质代谢情况，从而评估AQS在促进脂质代谢方面的潜在药理作用。随后，对比冷刺激前后棕色脂肪组织（BAT）表皮温度差异，检测肝脏和BAT组织中脂代谢相关基因的表达，以明确AQS的作用机制。结果：。网络药理学分析结果表明，AQS参与调控肥胖症过程中肝脏和脂肪组织功能。病理生化结果表明，AQS能减少不健康饮食引起的肝脏和脂肪组织的脂质积累。然而，AQS增加 BAT 的体积和重量，增强了小鼠的耐寒能力，表明AQS可促进以 BAT 为主导的机体产热。BAT组织中解偶联蛋白1（UCP1）介导的适应性产热和肝脏脂质代谢相关基因的水平在AQS治疗也有所升高，进一步证明了AQS可激活BAT产热功能，促进全身脂质代谢。结论：该研究为AQS通过调节BAT产热和肝脏脂质代谢以缓解肥胖症提供了科学依据，并为进一步探索天然活性成分在代谢性相关疾病中的应用提供了线索。
Abstract:
Objective: Cassiae Semen (CS, Juemingzi in Chinese) is a widely used traditional Chinese medicine with a variety of pharmacological effects. This study aimed to investigate the potential therapeutic effects and molecular mechanisms of anthraquinones of CS (AQS) for adiposity. Methods: The chemical components of the AQS were determined using high-performance liquid chromatography (HPLC). Network pharmacology analysis was used to predict potential anti-obesity targets of action for AQS. We constructed high fat with high sugar water diet-induced obese mice and observed their body weight and whole-body lipid metabolism to evaluate the efficacy of AQS in promoting lipid metabolism. Subsequently, the epidermal temperature at the brown adipose tissue (BAT) before and after cold stimulation was observed and the expression of lipid metabolism-related genes in the liver and BAT tissues was detected to clarify the mechanism of action of AQS. Results: Network pharmacology analysis showed that AQS was involved in the regulation of liver and adipose tissue function under obesity. Pathological and biochemical results showed that AQS reduced lipid accumulation in the liver and adipose tissue induced by an unhealthy diet. With the increase of cold tolerance, the volume and weight of BAT were increased by AQS, suggesting that it regulated the body heat production dominated by BAT. After AQS treatment, the levels of genes related to uncoupling protein1 (UCP1) mediated adaptive thermogenesis in BAT tissues and lipid metabolism in the liver were also increased, which further proved that AQS activated BAT function to promote lipid metabolism in the whole body. Conclusion: This study revealed the pharmacological effects of AQS, thereby providing a scientific basis for regulating BAT thermogenesis and liver lipid metabolism to alleviate obesity and providing clues for further exploring the application of natural active ingredients in the treatment of metabolismrelated diseases.
关键词:
肥胖;蒽醌类化合物;棕色脂肪组织;决明子;肝脏;脂质代谢;产热作用
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This work was supported by grants from the Fundamental Research Funds for the Central Universities (No. 2023-JYB-JBZD-046); National Key Research, Development Program on Modernization of Traditional Chinese Medicine (No. 2022YFC3502100); the National High-Level Talents Special Support Program; High-level traditional Chinese medicine key subjects construction project of National Administration of Traditional Chinese Medicine-Chinese Medicine Epidemic Disease (No. zyyzdxk-2023264).
Yijie Li a, Ruiyu Wu b, Xin Li b, Jianan Li a, Yinhao Zhang a, Yanbo Huang c, Guifang Fan b, Xiaojiaoyang Li a,?. Anthraquinones of Cassiae Semen alleviate lipid accumulation in obesity by regulating brown adipose tissue and liver function[J]. Chinese Herbal Medicines (CHM),2025,17(3):488-499
