Objective: Rhei Radix et Rhizoma has five types of products, namely, raw rhubarb (RR), wine rhubarb (WR), vinegar rhubarb (VR), cooked rhubarb (CR), and rhubarb charcoal (RC). However, Rhei Radix et Rhizoma is easily contaminated with fungi and mycotoxins if not harvested or processed properly. Here, we intend to analyze how microbiome assemblies and co-occurrence patterns are influenced by sampling locations and processing methods. Methods: High-throughput sequencing and internal transcribed spacer 2 (ITS2) were carried out to study the diversities (a- and b-diversity), composition (dominant taxa and potential biomarkers), and network complexitity of surface fungi on RR, WR, VR, CR, and RC collected from Gansu and Sichuan provinces, China. Results: The phyla Ascomycota and Basidiomycota; the genera Kazachstania, Malassezia, and Asterotremella; and the species Kazachstania exigua, Asterotremella pseudolonga, and Malassezia restricta were the dominant fungi and exhibited differences in the two provinces and the five processed products. The a-diversity and network complexity were strongly dependent on processing methods. Chao 1, the Shannon index, and network complexity and connectivity were highest in the CR group. The adiversity and network complexity were influenced by sampling locations. Chao 1 and network complexity and connectivity were highest in the Gansu Province. Conclusion: The assembly and network of the surface microbiome on Rhei Radix et Rhizoma were shaped by processing methods and sampling locations. This paper offers a comprehensive understanding of microorganisms, which can provide early warning for potential mycotoxins and ensure the safety of drugs and consumers.
关键词:
群体组装;真菌;加工方法;大黄;产地
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This study was supported by grants from the National Key R&D Plan (No. 2022YFC3501801, 2022YFC3501802 and 2022YFC3501804), Fundamental Research Funds for the Central Public Welfare Research Institutes (No. ZXKT21037, ZZ15-YQ-044, ZXKT22050 and ZXKT22001), Scientific Research Project of Hainan Academician Innovation Platform (No. YSPTZX202137 and SQ2021PTZ0052), and Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences (No. CI2023E002-Y-58).
Guangfei Wei a,, Xiao Chen b,, Guozhuang Zhang a, Conglian Liang c, Zhaoyu Zhang a, Bo Zhang d, Shilin Chen a, e, Linlin Dong a,*. Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations[J]. Chinese Herbal Medicines (CHM),2025,17(1):189-199
