Objective: Therapeutic angiogenesis has become a promising approach for treating ischemic heart disease (IHD). The present study aims to investigate the effects of Qishen Granule (QSG) on angiogenesis in myocardial ischemia (MI) and the potential mechanism. Methods: In vivo study was conducted on rat model of myocardial infarction. QSG was performed daily at a dose of 2.352 g/kg for four weeks. Cardiac function was assessed by echocardiogram and pro-angiogenic effects were evaluated by Laser Doppler and CD31 expression. Oxygen-glucose deprivation (OGD) was applied in cultured human umbilical vein endothelial cells (HUVECs). Cell viability, wound healing and tube formation assay were used to test functions of HUVECs. ELISA and Western blots were used to assess protein expressions of bone morphogenetic protein 2-delta-like 4-notch homolog 1 (BMP2-Dll4-Notch1) signaling pathway. Results: The results showed that QSG improved heart function, cardiac blood flow and microvessel density in myocardial ischemic rats. In vitro, QSG protected HUVECs by promoting the cell viability and tube formation. QSG upregulated bone morphogenetic protein-2 (BMP2) and downregulated delta-like 4 (Dll4) and notch homolog 1 (Notch1) expressions both in rats and HUVECs. Conclusion: QSG protected against MI by promoting angiogenesis through BMP2-Dll4-Notch1 pathway. BMP2 might be a promising therapeutic target for IHD.
关键词:
血管新生;骨形态发生蛋白2;Delta样配体4;心肌缺血;Notch同源体1;芪参颗粒
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This work was supported by the National Natural Science Foundation of China held by LL (No. 81904169) and Excellent Youth Foundation of BUCM held by DG (No. BUCM-2019-JCRC005).
Yiqin Hong a,, Hui Wang a,, Hanyan Xie a,, Xinyi Zhong a, Xu Chen b, Lishuang Yu b, Yawen Zhang b, Jingmei Zhang a, Qiyan Wang a, Binghua Tang a, Linghui Lu b,*, Dongqing Guo a,*. Qishen Granule protects against myocardial ischemia by promoting angiogenesis through BMP2-Dll4-Notch1 pathway[J]. Chinese Herbal Medicines (CHM),2025,17(1):139-147
