Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

doi:https://doi.org/10.1016/j.chmed.2023.10.003

Archive > Volume 16 Issue 2 > 2024,16(2):274-281. DOI:https://doi.org/10.1016/j.chmed.2023.10.003 Prev Next

黄芪桂枝五物汤通过抑制炎症和骨破损改善胶原诱导性关节炎小鼠模型症状的研究

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Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

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摘要:

目的：类风湿关节炎（RA）是一种慢性炎性和破坏性关节炎，其特征在于炎性浸润和骨破损。黄芪桂枝五物汤（HGWD）是中国传统中药复方，在临床上被应用于RA的治疗。本研究旨在探究HGWD对胶原诱导性关节炎（CIA）小鼠模型的治疗效果。方法：采用雌性DBA/1J小鼠建立胶原诱导关节炎模型。第一次免疫后第22天开始每天一次HGWD灌胃给药，持续四周。每五天测量小鼠体重、后爪厚度和临床症状评分。进行步态分析、组织病理学染色、酶联免疫吸附测定（ELISA）、超声成像和显微计算机断层成像以确定该模型中HGWD治疗对炎症和骨结构的影响。此外，通过RT-PCR和Western blot检测HGWD干预对Raw 264.7细胞相关炎症因子mRNA及蛋白质水平的表达情况。结果：HGWD可以减轻CIA小鼠关节炎症状，抑制炎症滑膜面积和血清中炎症因子的水平，抑制膝关节和踝关节的关节空间增大，减少破骨细胞数量，保护骨破坏，以及改善运动功。同时HGWD降低可以降低LPS诱导后Raw 264.7炎症因子mRNA的表达和p-NF-κb和IL-17的蛋白质表达水平。结论：这些结果表明HGWD具有抑制炎症，减少骨侵蚀和保持运动功能的效果。

Abstract:

Objective: Rheumatoid arthritis (RA) is a chronic inflammatory and destructive arthritis, characterized by inflammatory infiltration and bone destruction. Huangqi Guizhi Wuwu Decoction (HGWD) is traditional Chinese medicine, which has been applied in the treatment of RA in clinical. The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis (CIA) mouse model. Methods: DBA/1J female mice were used to establish the collagen-induced arthritis (CIA) model. HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immunization. The body weight, hind paw thickness and clinical score were measured every five days. Gait analysis, histopathological staining, enzyme-linked immunosorbent assay (ELISA), ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model. Moreover, Real-time PCR and Western blot analysis were used to detect inflammatory factors mRNA and protein levels after HGWD intervention in RAW 264.7 cells. Results: HGWD attenuated symptoms of arthritis, suppressed inflammatory synovium area and the serum levels of inflammatory factors, inhibited joint space enlargement in the knee and ankle joints, reduced numbers of osteoclasts, protected bone destruction, as well as improved motor function. HGWD decreased the expression of mRNA for inflammatory factors and the protein expression levels of p-NF-κB and IL-17. Conclusion: These results suggested that HGWD suppresses inflammation, attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.

关键词:

骨破损;胶原诱导性关节炎;黄芪桂枝五物汤;炎症;类风湿关节炎

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Project Supported:

This work was sponsored by National Natural Science Foundation (No. 81822050, 81920108032, 81904227), Shanghai ‘‘Science and Technology Innovation Action Plan” Medical Innovation Research Project (No. 21Y11921400), the Program for Innovative Research Team of Ministry of Science and Technology of China (No. 2015RA4002), ‘‘Innovation Team” Development Projects (No. IRT1270), Innovative Team Project of Scientific Research Project of Traditional Chinese Medicine of Shanghai Municipal Health Commission (No. 2022CX001), Shanghai TCM Medical Center of Chronic Disease (No. 2022ZZ01009), Jing’an District Health Research Project of Shanghai (No. 2022MS03).

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黄芪桂枝五物汤通过抑制炎症和骨破损改善胶原诱导性关节炎小鼠模型症状的研究

Original articles

Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

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