Traditional Chinese medicines (TCMs) possess a rich historical background, unique theoretical framework, remarkable therapeutic efficacy, and abundant resources. However, the modernization and internationalization of TCMs have faced significant obstacles due to their diverse ingredients and unknown mechanisms. To gain deeper insights into the phytochemicals and ensure the quality control of TCMs, there is an urgent need to enhance analytical techniques. Currently, two-dimensional (2D) chromatography, which incorporates two independent separation mechanisms, demonstrates superior separation capabilities compared to the traditional one-dimensional (1D) separation system when analyzing TCMs samples. Over the past decade, new techniques have been continuously developed to gain actionable insights from complex samples. This review presents the recent advancements in the application of multidimensional chromatography for the quality evaluation of TCMs, encompassing 2D-gas chromatography (GC), 2D-liquid chromatography (LC), as well as emerging three-dimensional (3D)-GC, 3D-LC, and their associated data-processing approaches. These studies highlight the promising potential of multidimensional chromatographic separation for future phytochemical analysis. Nevertheless, the increased separation capability has resulted in higher-order data sets and greater demands for data-processing tools. Considering that multidimensional chromatography is still a relatively nascent research field, further hardware enhancements and the implementation of chemometric methods are necessary to foster its robust development.
关键词:
化学计量学; 多维分离;离线二维液相色谱法;在线二维液相色谱法;质量控制;中药;在线二维液相色谱法
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We appreciate Zhijun Zhao (J&X Technologies) and Sifan Luo (Guangdong MS Institute of Scientific Instrument Innovation) for their scientific assistance in 2D-GC and ToF MS explanation. This work is financially supported by the Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization. Additional funding was provided by the Hunan Province Natural Science Fund (No. 2020JJ4569 and 2023JJ60378), and the Hunan Province College Students’ Innovation and Entrepreneurship Training Program (No. S202110530044 and S202210530048). The study has received approval from the university’s review board.
Xinyue Yang a,, Pingping Zeng a,, Jin Wen a, Chuanlin Wang a, Liangyuan Yao b, Min He a,*. Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches[J]. Chinese Herbal Medicines (CHM),2024,16(1):27-41
