Objective: Gentamicin (GM) is a commonly used aminoglycoside antibiotic, however, renal toxicity has limited its usage. The present study was designed to evaluate the ameliorative effect of Cistanche deserticola on GM-induced nephrotoxicity in rats. Methods: The nephrotoxicity in rats was induced by intraperitoneal administration of GM (100 mg/kg) for 10 consecutive days. Glomerular filtration rate, blood urea nitrogen, creatinine and kidney histopathology were detected to assess the GM-induced nephrotoxicity. The oxidative stress (catalase, superoxide dismutase, glutathione and malondialdehyde) was assessed. The inflammatory response (tumor necrosis factor-a, interleukin-6, myeloperoxidase and nuclear factor-kappa B) and apoptotic marker (Bax and Bcl-2) were also evaluated. Results: The results showed that water and 75% ethanol extracts of C. deserticola (named CDW and CDE, respectively) (100, 200 and 400 mg/kg) in combination with GM could recover the reduction of glomerular filtration rate and enhance the renal endogenous antioxidant capability induced by GM. The increase in the expression of renal inflammatory cytokines (tumor necrosis factor-a and interleukin-6), nuclear protein of nuclear factor-kappa B (p65) and the activity of myeloperoxidase induced by GM was significantly decreased upon CDW or CDE treatment. In addition, CDW or CDE treatment could decrease the Bax protein expression and increase the Bcl-2 protein expression in GM-induced nephrotoxicity in rats significantly. Conclusion: The study demonstrated that C. deserticola treatment could attenuate kidney dysfunction and structural damage in rats induced by GM through the reduction of inflammation, oxidative stress and apoptosis.
关键词:
凋亡;肉苁蓉;庆大霉素;炎症;肾毒性;列当科;氧化应激
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This work was supported by the National Key Research and Development Program of China (2018YFC1706300, 2018YFC17063003), the Key Research and Development Project of GanSu Province (20YF2FA019), the People’s Livelihood Special Project from Technical Innovation Guide Plan of Gansu Province (20CX4FK014), the Key Talent Project of Organization Department of Gansu Provincial Party Committee ([2022] Gan-71), the Talent Innovation and Entrepreneurship Project of Lanzhou (2017-RC115, 2020-RC-41).
Ruibin Bai a, Jingmin Fan a, Yanping Wang a, Yan Wang a, Xue Li a, Fangdi Hu a, b,c,*. Protective effect of Cistanche deserticola on gentamicin-induced nephrotoxicity in rats[J]. Chinese Herbal Medicines (CHM),2023,15(1):102-109
