Objective: To examine the protective effects of hydroxysafflor yellow A (HSYA) against the senescence of mesenchymal stem cells (MSCs) induced by D-galactose (D-gal) in vitro, and investigate the potential mechanism involved. Methods: Grouping experiment, Normal control (NC) group: conventional culture with complete medium; Senescence group: MSCs were cultured for 48 h with complete medium containing 10 g/L D-gal; HSYA group: on the basis of senescence induction, HSYA with the suitable concentration was used to protect MSCs. The key experimental indices associated with oxidative stress, inflammatory response, cell senescence, proliferation and apoptosis were measured through chemical colorimetry, b-galactosidase staining, EdU incorporation and flow cytometry, respectively. The relative quantity (RQ) of proteins related closely to cell proliferation, apoptosis, and NF-jB signaling were measured by Western blotting. Results: As compared with Senescence group, treatment with HSYA (120 mg/L) effectively ameliorated the adverse situation of MSCs. Oxidation stress and inflammation along with D-Gal induction was dramatically alleviated in MSCs; The b-Gal-positive staining indicated that MSC senescence was significantly mitigated; The proliferative capability of MSCs was significantly increased by up-regulating PCNA and inhibiting p16 expression; The anti-apoptotic effect on MSCs was exerted by down-regulating the RQ of cleaved Caspase-3 and Bax; The activity of NF-jB signaling in MSCs was notably suppressed through inhibiting phosphorylation of IKKb and p65. Conclusion: HSYA (120 mg/L) significantly delayed the D-Gal-induced senescence process in MSCs through attenuating inflammatory reaction and oxidative stress, and suppressing the activity of NF-jB signaling.
关键词:
细胞衰老;羟基红花黄色素;间充质干细胞
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This study was supported by Applied Basic Research Project of Zhangjiakou Science and Technology Bureau (No. 1911020D), General Project of Hebei North University (No. YB2018034), Morphology Experimental Teaching Center of Hebei Province (No. XTZX201901) and College Students’ Innovation and Entrepreneurship Training Program (No. S202110092005).
Xiaoqing Song *, Jinying Wang, Yu Zhang, Xinqian Du, Qibing Qian. Protective effect of hydroxysafflor yellow A on MSCs against senescence induced by D-galactose[J]. Chinese Herbal Medicines (CHM),2023,15(1):86-93
