Genome sizes of four important medicinal species in Kadsura by flow cytometry
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
摘要:
Abstract:
Objective: Dianjixueteng is a geoherb in Yunnan Province, the source plant of which is Kadsura interior. However, the formation of this geoherb is not clear in genetic mechanism, in which genome size is the first step that should be known on the genomic level. In this study we aimed to estimate the genome sizes of source plants of K. interior compared with three related herbs K. heteroclita, K. longipedunculata, and K. coccinea by flow cytometry (FCM). Methods: The genome sizes of K. interior, K. heteroclita, K. longipedunculata and K. coccinea, i.e., the source plants of K. interior and its relative medicinal materials, were estimated by FCM. The nuclei of K. interior were isolated using modified LB01 buffer, for the rest species, by the Galbraith’s buffer. Results: The genome sizes of K. interior, K. heteroclita, K. longipedunculata, and K. coccinea were 7.36, 7.12, 7.01, and 5.15 pg/1C, respectively. Genome size of K. interior had no significant variation with those of K. heteroclita and K. longipedunculata (P = 0.296), which were larger than that of K. coccinea. Conclusion: Genome size did not distinguish K. interior, K. heteroclita and K. longipedunculata, but did distinguish from K. coccinea, which lays the foundation for future studies on genetic mechanism of the geoherb formation.
关键词:
Keywords:
Project Supported:
We sincerely thank Mr. Zhi-liang Peng (Guihuashu Forest Farm of Fengqing Forestry Bureau) and Mr. Liang-yi Yang (Guihuashu Forest Farm of Fengqing Forestry Bureau) for collecting organisms of Kadsura species. We also thank Prof. Xian-chun Zhang (IBCAS: The Institute of Botany, Chinese Academy of Sciences) and Miss Si-qi Liang (IBCAS) for providing organisms of reference standards plants. We thank Mrs. Jin-dan Zhang from the Plant Science Facility of IBCAS for her excellent technical assistance on FCM. This study was financially supported by the National Natural Science Foundation of China (Grant No.81872965, 81703650).
Jing Xua, Xue-ping Weia, b,*, Jiu-shi Liua, b, Yao-dong Qia, b, Ben-gang Zhanga, b, Hai-tao Liua, b, Pei-gen Xiaoa, b. Genome sizes of four important medicinal species in Kadsura by flow cytometry[J]. Chinese Herbal Medicines (CHM),2021,13(3):
