UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius
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摘要:
Abstract:
Objective: In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from Carthamus tinctorius which contains diverse types of glycosides were mined. Methods: A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into Escherichia coli for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MSn. The structures of representative catalytic products were elucidated by MS and NMR. Results: UGT88B2 exhibited catalytic promiscuity and various patterns in glycosylation of flavonoids with high efficiency. Conclusion: A new glycosyltransferase named UGT88B2 was successfully mined and can be employed as enzymatic tools in glycosylation of flavonoids.
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This work is financially supported by the National Natural Science Foundation of China (No. 81573317).
Song-yang Suia, Rui-mingqian Guoa, Ke-bo Xieb, Lin Yanga. UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius[J]. Chinese Herbal Medicines (CHM),2020,12(4):
