Antioxidation and active constituents analysis of flower residue of Rosa damascen
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Objective: To make full usage of resource and turn waste into treasure, the chemical constituents and bioactivity were firstly investigated on Damask rose (Rosa damascena) flower residue (DRFR). Methods: DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR. Then, column chromatography was used to purify compounds from an antioxidation extract (DRFR-A), and the chemical structure was identified using NMR. The total phenolic acid content was measured by Folin-Ciocalteu colorimetric method, and the content of gallic acid of the indicator ingredient was detected by HPLC. Results: DRFR-A was found to show a high activity both on DPPH (IC50: 2.760 μg/mL) and ABTS (IC50: 2.258 μg/mL) compared to positive control VC. Ten compounds were isolated and identified as quercetin (1), kaempferol (2), gallic acid (3), protocatechuic acid (4), pyrogallic acid (5), 2-phenylethyl 3,4,5-trihydroxybenzoate (6), methyl gallate (7), p-hydroxybenzoic acid (8), p-hydroxyphenethyl alcohol (9) and astragalin (10) from DRFR-A. Among them, pyrogallic acid, 2-phenylethyl-3, 4, 5-trihydroxybenzoate, p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time. The content of total phenolic acids and gallic acid, main ingredient in DRFR-A was determined as 63.73% and 24.67%, respectively. Conclusion: This study provides a reliable data and lays the foundation for the development and utilization of rose residue, and hence for the full utilization of rose resources.
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The authors wish to thank associate professor Cheng-xue Pan from Zheng-zhou University for his assistance in the plants identification and authentications. This project was supported financially by the National Natural Science Foundation of China (Grant No. 331/313400531021).
Wen‐ya Liu a, Lin‐yu Chen a, Ying‐ying Huang a, Ling Fu a, b, Le‐yuan Song a, Yun‐yu Wang a, Zhe Bai a, Fang‐feng Meng a, Yue‐feng Bi a, b. Antioxidation and active constituents analysis of flower residue of Rosa damascen[J]. Chinese Herbal Medicines (CHM),2020,12(3):
