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[摘要]
目的 从枇杷Eriobotrya japonica叶中分离纯化得到枇杷叶多糖,并对其进行相对分子质量和单糖组成分析以及结构的初步鉴定。方法 枇杷叶经水提、醇沉、冷冻干燥得到枇杷叶多糖(PEL)组分,经DEAE-Sepharose Fast Flow离子柱和Sephacryl S 500 High-Resolution凝胶柱色谱进行分离纯化得均一组分(PEL60-A)。采用高效液相色谱、气质联用确定其纯度、相对分子质量和单糖组成。结果 枇杷叶多糖PEL60-A相对分子质量为2.69×105;单糖组成主要是L-鼠李糖、L-岩藻糖,并含有少量D-木糖、D-甘露糖、D-葡萄糖和D-半乳糖,其物质的量比为0.78∶1.00∶0.15∶0.09∶0.11∶0.19。结论 研究枇杷叶多糖分离纯化及其单糖组成,对其结构进行了初步鉴定,为枇杷叶精细加工提供重要的科学理论依据。
[Key word]
[Abstract]
Objective To determine the relative molecular weight (Mw), monosaccharide composition, and structures of polysaccharides from the leaves of Eriobotrya japonica. Methods The polysaccharides (PEL60) were extracted from the leaves of E. japonica with hot water, followed by precipitating with ethanol, freezing, and drying. PEL60-A was purified by DEAE-Sepharose Fast Flow ion column and Sephacryl S 500 High-Resolution gel column chromatography from PEL60 of the leaves of E. japonica. The purity and molecular weight of PEL60-A was detected by high performance liquid chromatography (HPLC), and the monosaccharide composition and molecular ratio of the PEL60-A was analyzed by gas chromatography-mass spectrometry (GC-MS). Results The PEL60-A, with Mw of 2.69×105. The composition of monosaccharide was mainly L-rhamnose and L-fucose and a small amount of D-xylose, D-mannose, D-glucose, and D-galactose with the molar ratio of 0.78︰1.00︰0.15︰0.09︰0.11︰0.19. Conclusion The study on isolation, purification, and preliminary identification of monosaccharide composition from leaves of E. japonica provided the important scientific theoretical basis for the fine processing of the leaves of E. japonica.
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[基金项目]
国家自然科学基金面上项目(31671813);浙江省自然科学基金资助项目(LY17C200017)