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[摘要]
目的 研究干燥前水洗对丹参Salvia miltiorrhiza活性成分量的影响, 为规范丹参产地加工方法提供依据。方法 将丹参鲜药材采取不洗、冲洗、浸洗、搓洗等方法处理, 采用HPLC法测定其活性成分量的变化, Diamonail C18色谱柱(250 mm×4.6 mm, 5 μm), 乙腈-0.026%磷酸溶液梯度洗脱, 体积流量为1.0 mL/min, 检测波长为286和270 nm。结果 水洗后, 迷迭香酸、丹酚酸B、隐丹参酮、丹参酮I、丹参酮IIA 5种活性成分量均有所降低, 水洗力度越大, 质量分数降低幅度越大, 搓洗丹参中的活性成分量最低。结论 水洗使丹参活性成分遭受损失, 应尽量采用冲洗方式, 既要减少与水接触的时间, 又要避免药材间摩擦及人工搓揉。
[Key word]
[Abstract]
Objective To research the influence of different washing methods before drying on the contents of active components in Salvia Miltiorrhizae Radix et Rhizoma, and provide the basis for standard original processing methods of Salvia miltiorrhiza. Methods The fresh herbs of S. miltiorrhiza were processed with different methods including no washing, water flushing, immersion cleaning, and scrubbing in water. HPLC was applied to determine the contents of active components, with Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), acetonitrile-0.026% phosphoric acid for gradient elution, flow rate of 1mL/min, and detection wave length of 286 and 270 nm. Results The contents of rosmarinic acid, salvianolic B, crypotanshinone, tanshinone I, and tanshinone IIA were all significantly decreased in S. miltiorrhiza processed with different washing methods. The contents of active components were reduced with the increasing of washing intensity, of which the lowest contents were found in scrubbing group. Conclusion Washing with water can make loss of active components, and flushing may be a more suitable method. In order to decrease the content loss of active components, contact time with water must be controlled as short as possible and violent friction among medicinal materials and artificial rubbing must be avoided.
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[基金项目]
国家科技重大专项课题(2012ZX09304006);山东省科技发展计划项目“山东道地药材资源质量控制科技平台建设”课题(2008GG2NS02022)