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[摘要]
目的 通过对桂林地区的银杏叶Ginkgo Folium一年内的总黄酮苷与总内酯量检测, 分析总黄酮和总内酯的比例变化规律, 找到最佳"酮酯比", 为确定桂林地区银杏叶药材的最佳采收期提供科学的实验依据。方法 使用回流提取法提取样品中总黄酮苷和总内酯, 使用HPLC法对其进行定量分析, 色谱柱为Ultimate XB-C18柱(250 mm×4.6 mm, 5 μm);总黄酮测定采用甲醇-0.4%磷酸溶液(48:52)为流动相;测波长360 nm;体积流量1.0 mL/min。总内酯测定以甲醇-四氢呋喃-水(25:10:65)为流动相;蒸发光散射检测器检测;体积流量0.6 mL/min。结果 桂林地区产银杏叶药材, 在1年内不同采收期银杏叶总黄酮苷和总内酯量差异较大, 但8月份2种有效部位量均较高, 二者量比例最小。结论 8月份为桂林地区银杏叶的最佳采收季节, 为指导桂林产银杏叶的采收提供实验依据。
[Key word]
[Abstract]
Objective There are abundant Chinese medicinal herb resources in Guangxi province. Especially, there are lots of Ginkgo Folium in the north. To detect the contents of the total flavonoid glycoside and lactone in Ginkgo Folium and to find out their best concentration ratio during the year. Methods The reflux method was used for the distillation of the total flavonoid glycoside and lactone in Ginkgo Folium and HPLC was used for quantitative analysis. The contents of the total flavonoid glycoside and lactone in Ginkgo Folium were determined on a Ultimate XB-C18 (250 mm × 4.6 mm, 5 μm) analytical column with different mobile phases and detectors. Results The concentration ratio of the total flavonoid glycoside and lactone in Ginkgo Folium in August was the smallest and their contents were high. Conclusion There is significant difference between total flavonoid glycoside and lactone content in Ginkgo Folium from different picking periods during the year, and the concentration ratio of the two compositions in August is the smallest with high contents, which can be used to provide the basis for guiding the farmer to collect Ginkgo Folium in August.
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