目的 研究天舒胶囊对电刺激大鼠上矢状窦区硬脑膜诱导偏头痛模型的影响，并探讨其作用机制。方法 雄性SD大鼠，脑部电极植入术后，ig给予天舒胶囊（1.08、0.36、0.18 g/kg），阳性药对照组ig琥珀酸舒马普坦片（9.72 mg/kg），除对照及假手术组外，行电压10 V、频率3 Hz、脉宽0.25 ms、时间1 h电刺激，对照组不予手术，并于刺激0、30、60 min进行眼眶采血，采血后取脑组织。采用放射免疫法检测血浆和脑组织中降钙素基因相关肽（CGRP）、P物质和去甲肾上腺素（NE）水平。结果 假手术组与对照组比较大鼠脑组织中CGRP、P物质和NE水平均无显著性差异；与假手术组相比，模型组大鼠脑组织中CGRP明显升高、P物质和NE水平明显降低（P<0.05），提示模型成功。与模型组相比，天舒胶囊可以显著提高大鼠血浆和脑中NE水平（P<0.05），降低CGRP水平（P<0.05），阳性药舒马普坦可以明显降低血浆中CGRP水平（P<0.05）。结论 天舒胶囊对电刺激上矢状窦区硬脑膜大鼠脑部损伤具有显著改善作用，其作用机制之一为调节神经递质释放。

Objective To study the effect of Tianshu Capsule on the rat dura mater model of superior sagittal sinusin following electrical stimulation and discuss its mechanism. Methods Male SD rats after brain electrode implantation were started on Tianshu Capsule at the doses of 1.08, 0.36, and 0.18 g/kg. Sumatriptanm Tablets at the dose of 9.72 mg/kg were ig given as a positive drug. Except control and Sham groups, the electrical stimulation was carried out in a voltage of 10 V, frequency of 3 Hz, pulse width of 0.25 ms, and time of 1 h. No operation was taken in the control group. The orbital blood was taken after 0, 30, and 60 min of the stimulation than taking brain tissue. The contents of calcitonin gene-related peptide (CGRP), substance P, and norepinephrine (NE) in plasma and brain tissue were detected by radio immunoassay. Results Compared with the control group, there was no significant difference of CGRP, substance P, and NE contents in brain tissue of rats in the Sham group. Compared with the Sham group, CGRP in the brain tissue of rats in normal group was increased obviously, while substance P and NE levels were significantly decreased (P < 0.05). Comapred with the model group, the Tianshu Capsule could increase the content of NE in plasma and brain tissue (P < 0.05) while decrease CGRP level (P < 0.05). And Sumatriptanm Tablets could reduce the content of CGRP in plasma (P < 0.05). Conclusion The Tianshu Capsule could significantly improve the damage of rat dura mater of superior sagittal sinusin following electrical stimulation by releasing neurotransmitter, which may be the mechanisms of migraine treatment.

国家科技部重大新药创制（2011ZX09401-097）