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[摘要]
目的 建立虎柏烧伤酊中大黄素、虎杖苷和盐酸小檗碱定量测定方法。方法 采用Eclipse XDB-C18(150 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,体积流量1.0 mL/min,检测波长265 nm(盐酸小檗碱)、306 nm(大黄素和虎杖苷),柱温30 ℃。结果 大黄素在6.3~200.0 ng、虎杖苷在9.6~308.0 ng、盐酸小檗碱在5.0~160.0 ng有良好的线性关系,样品中大黄素、虎杖苷和盐酸小檗碱的平均加样回收率分别为99.91%、99.51%和99.16%,RSD分别为1.12%、0.84%和1.17%。结论 本法结果准确,精密度及重复性较好,可作为虎柏烧伤酊的质量控制方法。
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[Abstract]
Objective To establish a method for determining the contents of emodin, polydatin, and berberine hydrochloride in Hubai Burrn Tincture. Methods The samples were separated on an Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) column by a gradient elution using acetonitrile and 0.1% phosphoric acid aqueous solution as mobile phase at a flow rate of 1 mL/min. The eluate was detected by double wavelengths: 265 nm for berberine hydrochloride and 306 nm for emodin and polydatin. Column teperature was set at 30 ℃. Results The linear ranges of emodin, polydatin, and berberine hydrochloride were 6.3—200.0, 9.6—308.0, and 5.0—160.0 ng. The average recovery rates of emodin, polydatin, and berberine hydrochloride were 99.91% (RSD = 1.12%), 99.51 % (RSD = 0.84%), and 99.16% (RSD = 1.17%). Conclusion This method is accurate, specific, and reliable for the quality control of Hubai Burrn Tincture.
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