目的 建立HPLC同时测定凉血通瘀颗粒中芍药苷、丹皮酚、芦荟大黄素、α-细辛醚、大黄素、大黄酚、大黄素甲醚7种成分的方法。方法 色谱柱为Lichrospher C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为乙腈（A）-0.1%磷酸水溶液（B），梯度洗脱：0～18 min，15% A，体积流量0.8 mL/min；18～20 min，15%～42% A，体积流量0.8 mL/min；20～54 min，42% A，体积流量0.8 mL/min；54～56 min，42%～70% A，体积流量1.0 mL/min；56～80 min，70% A，体积流量1.0 mL/min；检测波长254 nm，柱温25 ℃。结果 7种成分均能达到基线分离，各成分均有较宽的线性范围和良好的线性关系，加样回收率在95%～105%。结论 本法准确、灵敏、可靠、重复性好，可用于凉血通瘀颗粒的质量控制。

Objective To develop an HPLC method for simultaneous determination of paeoniflorin, paeonol, aloeemodin, α-asarone, emodin, chrysophanol, and physcion in Liangxue Tongyu Granule. Methods The chromatographic separation was achieved on an Lichrospher C₁₈ (250 mm × 4.6 mm, 5 μm) column with acetonitrile (A) - 0.1% phosphoric acid (B) as mobile phases for gradient elution, 0—18 min, 15% A at the flow rate of 0.8 mL/min; 18—20 min 15%—42% A at the flow rate of 0.8 mL/min; 20—54 min, 42% A at the the flow rate of 0.8 mL/min; 54—56 min, 42%—70% A at the flow rate of 1.0 mL/min; and 56—80 min, 70% A at the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was set at 254 nm. Results The results showed that seven active components were well separated and showed good linearity. The average recoveries were between 95%—105%. Conclusion The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Liangxue Tongyu Granule.

江苏高校优势学科建设工程资助项目（2011-2015）；江苏省科技厅科技支撑计划（BE2011849）；江苏省“六大人才高峰”项目（2009）；江苏省“青蓝工程”科技创新团队支持计划[苏教师（2008）30号]；南京中医药大学科技创新风险基金项目计划（CX200904）