目的 研究冬凌草甲素注射剂诱导人胃癌SGC-7901细胞凋亡及与细胞内Ca²⁺浓度（[Ca²⁺]_i）的关系。方法 MTT法检测冬凌草甲素注射剂对SGC-7901细胞的细胞毒活性；倒置显微镜观察冬凌草甲素注射剂对细胞形态学的影响；流式细胞术测定细胞凋亡率；激光共聚焦显微术检测SGC-7901细胞[Ca²⁺]_i的变化；JC-1单染，激光共聚焦显微术检测SGC-7901细胞中线粒体膜电位的变化。结果 冬凌草甲素注射剂对SGC-7901细胞的IC₅₀为21.74 μmol/L；作用48 h后，细胞生长密度变疏，细胞皱缩，其中冬凌草甲素注射剂高浓度组大部分细胞破碎。冬凌草甲素注射剂5.5、11、22 μmol/L作用24 h后SGC-7901细胞凋亡率分别为7.07%、18.57%、22.96%；钙离子荧光强度高于对照组；线粒体膜电位荧光强度显著低于对照组。结论 冬凌草甲素注射剂通过升高SGC-7901细胞[Ca²⁺]_i诱导细胞凋亡。

Objective To study the apoptosis of Oridonin Injection against human gastric carcinoma SGC-7901 cell line and its relationship with intracellular calcium concentration([Ca²⁺]_i ). Methods The cytotoxicy of Oridonin Injection against SGC-7901 cells was evaluated by MTT assay. Morphology of SGC-7901 cells was observed by inverted microscope. Cell apoptosis rate was measured by flow cytometry. [Ca²⁺]_i of SGC-7901 cells and mitochondrial membrane potential (MMP)were determined by laser scanning confocal microscope. Results IC₅₀ of Oridonin Injection to SGC-7901 cells was 21.74 μmol/L. Cell density decrease, cell shrinkage, and cell breakage in high-dose group were observed 48 h after administration. The apoptosis rate values of 5.5, 11, and 22 μmol/L Oridomin Injection groups 24 h after administration were 7.07%, 18.57%, and 22.96%, respectively. Fluorescence intensity (FI) values of [Ca²⁺]_i were significantly higher than those in control group. And FI values of MMP were significantly lower than those in control group. Conclusion Oridonin Injection could induce cell apoptosis by increasing [Ca²⁺]_i of SGC-7901 cells.

黑龙江省新世纪优秀人才支持计划（1251-NCET-019）；黑龙江省研究生创新基金项目（YJSCX2011-163HLJ）