[关键词]
[摘要]
目的以知母种子为试验材料,对知母的组织培养进行了初步研究,以期建立知母的再生体系。方法采用植物组织培养和单因子试验的方法对知母无菌体系的建立、分蘖芽的增殖、分蘖愈伤组织的诱导和再分化以及再生苗的移栽进行研究。结果 知母种子最佳的消毒方式是75%酒精处理30 s后用0.1%HgCl2处理15 min;知母分蘖芽增殖的最佳培养基是MS+KT 1 mg/L+NAA 0.5 mg/L;知母分蘖愈伤组织诱导的最佳培养基是MS+KT 2 mg/L+NAA 0.5 mg/L;知母愈伤组织再分化的最佳培养基是MS+KT 2 mg/L+NAA 0.1 mg/L;知母愈伤组织再生芽最佳的生根培养基是1/2MS+NAA 0.5 mg/L;知母试管苗最佳的移栽基质是腐殖土。结论 本实验建立了知母的再生体系,为知母试管苗的工厂化生产奠定了技术基础。
[Key word]
[Abstract]
Objective The tissue culture of Anemarrhena asphodeloides was preliminarily studied to establish A.asphodeloides regeneration system.Methods The establishment of A.asphodeloieds sterile system,tiller bud proliferation,tiller callus induction and its re-differentiation as well as transplanting of regenerated plantlets were studied by plant tissue culture and single factor test method.Results The best disinfection way of A.asphodeloides seeds was firstly dealt with 75% ethanol for 30 s and then dealt with 0.1% HgCl2 for 15 min;The best medium of bud proliferation for A.asphodeloides tillers was MS+KT 1 mg/L+NAA 0.5 mg/L;The best medium of A.asphodeloides tiller callus induction was MS+KT 2 mg/L+NAA 0.5 mg/L;The best medium of A.asphodeloides tillers callus redifferentiation was MS+KT 2 mg/L+NAA 0.1 mg/L;The best rooting medium of A.asphodeloides callus regeneration buds was 1/2 MS+NAA 0.5 mg/L;The best transplanting substrate of A.asphodeloides plantlets was humus soil.Conclusion The regeneration system of A.asphodeloides is established,which provides a technological basis for factory production of A.asphodeloides plantlets.
[中图分类号]
[基金项目]
上饶师范学院2010-2011年度院级科技项目