目的探讨灵芝孢子油对N-甲基-N-亚硝脲(MNU)诱导的大鼠视网膜光感受器细胞变性动物模型的治疗作用,为防治视网膜变性提供一种有价值的现代化中药。方法大鼠随机分为5组,对照组、灵芝孢子油治疗组、二十二碳六烯酸(DHA)治疗组、灵芝孢子油+DHA治疗组和模型组,在造模前2 d用不同的药物ig给药,第3天采用40 mg/kg MNU单次剂量ip造模,造模后继续ig给药至第10天,造模后1、3、5、7、10 d进行视网膜电图(ERG)检查,并取眼球进行眼病理检查。结果不同用药组各时间点的b波振幅明显高于模型组(P<
0.05、0.01);
灵芝孢子油组的a波振幅明显高于模型组(P<
0.05),但与DHA组和灵芝孢子油+DHA组相比较,无明显差异(P>
0.05)。眼病理结果,不同用药组在各个时间点与相应的模型组比较,MNU诱导的大鼠视网膜光感受器损伤均明显减轻(P<
0.05)。结论灵芝孢子油和DHA可减轻MNU对大鼠视网膜光感受器细胞的损伤程度,促进MNU诱导的大鼠视网膜光感受器细胞损伤的功能恢复。

Objective To approach the therapeutical effect of the oil in Ganoderma lucidum spores(OGLS) on the retina photorecetor damage of rats induced by N-methyl-N-nitrosourea(MNU) and look for a kind of effective modern Chinese materia medica for preventing and treating retinitis pigmentosa.Methods Sprague-Dawley(SD) rats(150) were randomly divided into five groups: normal group,OGLS group,docosahexaenoic acid(DHA) group,OGLS combined with DHA group,and model group.After two days of ig administration with different medicines,besides the rats in normal group,the rats in the other groups were received a single dose of MNU(40 mg/kg) by ip injection in day 5. At different times,1,3,5,7,and 10 d,six rats in the different groups were examined with electroretinogram and their eyes were enucleated after sacrificing rats for pathological examination.Results The results from ocular eletroretinogram showed that the b wave amplitude of different medical treated groups was obviously higher than those of MNU model group at various time points,P0.05,or P0.01.The a wave amplitude of OGLS group was higher than those of MNU model group,P0.05,but it was not significant by comparing with DHA group and OGLS combined with DHA group, P0.05.The results from ocular pathology showed that the retinal photoreceptor damage of rats induced by MNU was obviously relieved in the different groups at various time points,compared with MNU model group,P0.05.Conclusion The final results suggest that OGLS and DHA could relieve retinal photoreceptor damage of rats induced by MNU and promote retinal functional recovery.

教育部博士点基金新教师项目(20070558281)