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[摘要]
目的研究羟基红花黄色素A(HSYA)与牛血清白蛋白(BSA)和卵清蛋白(OVA)的偶联方法,获得具有免疫原性的羟基红花黄色素A人工抗原。方法通过偶联物透析液的颜色变化、紫外图谱、红外扫描图谱及动物免疫试验来鉴定制备的人工抗原。结果HSYA-BSA人工抗原在紫外扫描中表现出HSYA和BSA的特征吸收峰;在红外扫描中HSYA-BSA具有HSYA特征官能团的吸收峰和氨基酸的特征吸收峰;间接ELISA检测结果显示产生抗HSYA的特异性抗体,血清效价可达1:3 200;标准曲线方程计算得BSA和HSYA的结合率是1:50。结论通过直接偶联法成功制备出具有免疫原性的羟基红花黄色素A人工抗原。
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[Abstract]
Objective To discuss the protective effect and mechanism of madecassoside on mice with acute liver injury induced by CCI‘.Methods Mice models of acute liver injury were provided withmadecassoside at different doses(3,10,and 30 mg/kg)separately.Serum function indexes including alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and contents of N0 in liver tissue were measured according to the instruction of test kits.Westernblotting was used for the analysis of protein expression of induced nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)in liver tissue.Some liver tissues were dyed with HE and histo pathological changes in liver were observed under light microscope.Results The levels of ALT and AST in serum and the content of NO in liver tissue were reduced significantly by madecassoside in mice with acute liver injury.Meanwhile,the protein expression of iNOS and COX-2 in liver tissue was also inhibited by madecassoside with histopathological changes of liver more improved than that in model group.Conclusion Madecassoside iS effective in amelioration of CCl4-induced hepatotoxicity.The effect may be due to its antinflammatory activity associated with lessincrease in hepatic iNOS and COX-2 protein content.
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[基金项目]
科技部省部共建新疆生物资源基因工程重点实验室开放基金资助(XJDX0201-2006-04)