[关键词]
[摘要]
目的 研究獐牙菜属药用植物叶上表皮细胞的形态差异,为本类药材的鉴别提供新的方法 。方法 采用常规技术制片,用计算机细胞分析系统HPIAS-1000对表皮细胞形态进行二值化处理,测定其上表皮细胞垂周壁弯曲程度即形态因子(SFC)和细胞排列方式即Feret’s直径比(SLF)。分别对12种獐牙菜属药用植物含不同产地的21种材料进行了测定,并进行了方法 学考察。结果 显微镜放大倍数组合20×10下,软件系统精密度和重现性良好,不同植株茎上第3节叶片中段主脉与第1侧脉(弧行脉序)之间上表皮细胞形态在种内最为稳定,不同种植物的SFC、SLF值有显著差异,每种都有其相对恒定值。结论 计算机细胞分析系统HPIAS-1000用于药用植物叶片表皮细胞的形态分析鉴定具有简便、快速、准确、实用的特点,为全草和叶类药材的鉴定提供了一种新途径。
[Key word]
[Abstract]
Objective? To study the shape differences of upper epidermal cells in leaves of medicinal plants of Swertia L. and establish the new method for identifying them. Methods? The epidermis were mounted in ordinary technique, and then scanned and binarized through HPIAS-1000 image analytic system. The waveness of the anticlinal walls (SFC) and the ratio of the Ferets diameter (SLF) of upper epidermal cells were detected. The leaf epidermal cells of 21 kinds of raw materials in 12 plants of Swertia L. from various regions were examined. Results? The precision and reproducibility of software system were good, and the shape characters of the upper epidermal cells of the middle lamina in the third node arising from ground are the stablest inside the same species by the magnification 20×10 under microscope. The SFC and SLF of the upper epidermal cells of the same species of plants are relatively constant, conversely that of different species of plants are obviously different and distinguishable from each other. Conclusion?? The proposed method is a useful technique for identifying traditional Chinese medicinal materials originated from herbs and leaves of plants. HPIAS-1000 is simple, rapid, accurate, and practical for shape analysis of upper epidermal cells of plant leaf.
[中图分类号]
[基金项目]
国家自然科学基金项目资助(30271590)