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[摘要]
目的建立木蝴蝶中黄酮类化合物的HPLC-ES I-M S分析方法,鉴定木蝴蝶中的黄酮类化合物。方法色谱柱:Hypers il C18(250 mm×4. 6 mm,5μm);流动相:0. 5%甲酸水溶液(A)、乙腈(B),梯度洗脱;紫外检测波长范围:195~400 nm;采用正离子检测模式。结果木蝴蝶中的6个黄酮类化合物获得了良好的分离与检测。通过与对照品的保留时间、正离子质谱比较鉴定和测定黄芩苷和白杨素的量,同时根据正离子质谱数据和文献数据分析推断了4个黄酮化合物。结论本方法准确快速,适合木蝴蝶中黄酮类化合物的鉴定,可用于木蝴蝶原药材的质量控制。
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[Abstract]
Objective To establish an HPLC-ESI-MS method for simultaneous analysis attd determination of flavone in the seeds of Oroxylum indicum. Methods The separation was performed by Hypersil C18column(250 mm×4. 6 mm,5μm)with a mobile phase of formic aeid aqueous solution(A)and acetonitrile(B)0. 5%. The flow rate was 1. 0 mL/min and detection wavelength was at 195-400 nrrl. The samples wete analyzed in positive mode. Results Six flavones in the seeds of 0. indicum could be separated in one run,Baicalin,ehrysin-7-glueuronide,oroxylin A,and baicalin-7-O-glucoside had been tentatively identifled by ESI-MS and reference data. Conclusion Besides quantification of the components,this new method surpasses previously published ones in product quality control with accurate and rapid advantage,and provides the HPLC chromatographic fingerprints of biological active components in the seeds of 0. indicum.
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