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[摘要]
目的 建立测定酸枣仁汤中棘苷和阿魏酸含量的 RP- HPL C分析方法。方法 色谱柱:Hypersil C18(2 0 0mm× 4.6 m m,5 μm),棘苷流动相为乙腈-1%冰醋酸水溶液 (17:83),检测波长 334nm;阿魏酸流动相为乙腈-1%冰醋酸水溶液 (16:84 ),检测波长 320 nm,内标物为对羟基苯甲醛。结果 棘苷和阿魏酸分别在 0.960~13.44μg/mL (r=0.9998)和 2.04~27.2 μg/mL (r=0.9997)与峰面积线性关系良好。平均回收率分别为 98.4%和98.8%,RSD分别为 2.5%和 2.8%。结论 本法操作简便、快速、准确,可用于酸枣仁汤中棘苷和阿魏酸的含量测定
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[Abstract]
Object To develop an RP-HPLC method for determination of spinosin and ferulic acid in Suanzaoren Decoction (SZRD). Methods Hypersil C 18 column was used. The mobile phase consisted of acetonitrile-water with 1% glacial acetic acid (17:83) for spinosin and (16:84) for ferulic acid. Detection wavelengths were set at 334 and 320 nm, respectively. The internal standard was p-hydroxybenzaldehyde for ferulic acid. Results The linear ranges for spinosin and ferulic acid were 0.960-13.44 μg/mL (r=0.999 8) and 2.04-27.2 μg/mL (r=0.999 7), respectively. The recoveries for them were 98.4% and 98.8%, respectively. The RSD were 2.5% and 2.8%, respectively.Conclusion This method is simple, rapids and accurate, and can be used for the determination of spinosin and ferulic acid in SZRD.
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