目的 建立和优化黄芩组织培养克隆化快速繁殖技术,为保护黄芩的野生资源,发展人工资源和探讨育种新途径奠定基础。方法 在组织培养条件下进行黄芩愈伤组织的诱导、分化,试管苗复壮和生根等一系列技术优化试验。结果 黄芩试管苗的节、节间都很容易诱导出愈伤组织,诱导率均达 10 0 %,而叶片的愈伤组织诱导率低,PP333对黄芩试管苗的生长有着显著的矮壮作用。结论 黄芩试管苗的节是诱导愈伤组织的理想外植体,在培养基中适当添加 PP333能显著改善试管苗的素质 ;PP333与激素的配合使用,能十分有效地调控黄芩愈伤组织的分化、试管苗的生长与生根,并能显著提高移栽成活率。

Object To establish and optimize the technology of clonig rapid propagation by tissue cul-ture in Scutellaria baicalensis Georgi for the protection and development of nature resource as well as seek-ing for new breeding way of S. baica lensis by biotechnology.Methods Research on the optimized tech-nology of tissue culture including callus inducing, differentiation, plant let propagation, and rooting were designed.Results Callus could be induced from nodes and in ternodes easilywith 100% inducing rate com-pared with that from leaves. PP333 has significant stocky effect to the plant let s. Conclusion The nodes are good explant source in inducing callus. PP333 in certain concen tration w hich is added to culture media could improve and regulate the differentiation of callus and growth of plant lets as well as increase the sur-vival rate of cultivated seeding.

国家中医药局重点科研项目 (97A302)