目的 研究草问荆总生物碱 (TAEP)抑制中枢神经系统的作用机制。方法 采用高效液相色谱 -电化学检测法 (HPL C- EC)及 HPL C- UV法测定大鼠脑内单胺类神经递质含量。结果 TAEP对大鼠纹状体的单胺类神经递质的含量具有显著的降低作用,同时升高纹状体单胺类递质中性及酸性代谢产物的含量 ;对大鼠前脑边缘区的单胺类递质具有显著的降低作用,同时显著升高边缘区单胺代谢物 5 -羟吲哚乙酸 (5 - HIAA ),高香草酸 (HVA)的含量。但对于 3,4 -二羟基苯乙醇 (DHPG)的升高作用无统计学意义。结论 TAEP具有与利血平相似的单胺排空作用,是 TAEP对中枢神经系统镇静安定的作用机制。

Object To reveal the mechanism of inhibitory action on the central nervous system (CNS) of total alkaloids of Equisetum pratense Ehrh. (TAEP). Methods Contents of monoamine neurotransmitters in brain of rats were determined by HPLC-EC and HPLC-UV. Results TAEP can significantly decrease the contents of monoamine neurotransmitter in the straite body and the bordering area of rat forebrain, increase the contents of neutral and acidic metabolites in the straite body, and increase the contents of 5-HIAA and HVA of the monoamines in the bordering area of rat forebrain. But its raise of DHPG showed no statistical significance. Conclusion TAEP has an evacuating action of monoamine similar to Reserpine. This may be considered as one of the mechanisms of sedative and tranquilizing effect CNS.

黑龙江省科委自然科学基金资助项目(D01-05)