目的 采用 MEKC和 HPL C法对黄芩多倍体进行了黄芩苷含量的测定,为大量样品的检测提供可靠的方法。方法 对 MEKC法和 HPL C进行方法学考察的基础上,测定了黄芩中黄芩苷的含量,并进行数据的比较和相关性分析。结果 两种方法的测定结果基本一致,其中采用 HPL C法测定的结果偏高 1%～ 3%,但两种方法测定的数据之间差异并无显著性,且相关系数较高。结论 两种方法均可以应用于黄芩苷含量测定,但是 MEKC法分析样品速度快、时间短、试剂费用低廉,如果仪器条件许可,MEKC法更适合于大量黄芩样品的测定。

Object To determine the baicalin content in skullcap polyploid by MEKC and HPLC, and to provide the reliable method used to determine a large number of samples. Methods On the basis of methodology of MEKC and HPLC, the baicalin in Scutellaria baicalensis Georgi. was determined, the data and relevant analysis were compared. Results The data of the content determined by HPLC was similar to that by MEKC, but the former showed slightly higher 1%-3%. There was no significant variation between the two Methods with higher correlation coefficient. Conclusion Both the above two Methods could be accurately used to determine the baicalin in S. baicalensis. The MEKC has some advantages, such as quick, save time with lower solvent cost, and is suitable for the large number of samples in the determination of baicalin of S. baicalensis.

国家中医药管理局重点课题项目(972095)