[关键词]
[摘要]
目的 建立 HPL C测定葛根芩连汤各配伍组合中葛根素含量的方法,研究配伍对葛根素含量的影响。方法采用 L8(2 7)正交设计及统计软件 SPSS10 .0统计分析,以 HPL C测定葛根素的含量。结果 黄芩、黄连、甘草对方中葛根素含量的影响差异不存在显著性,两两交互作用差异不具有显著性。实验中观察到凡是黄连与葛根的配伍组合中皆有沉淀产生,并且发现沉淀中含有一定量的葛根素。结论 黄芩、黄连、甘草对葛根素的含量没有显著性影响。含黄连和葛根配伍煎液产生的沉淀中含葛根素可能与 p H值的改变有关。
[Key word]
[Abstract]
Object To set up the quantitative method for determining the puerarin in Gegen Qinlian Decoction (GQD) by RP-HPLC and to study the change of puerarin contents in various combinations of GQD. Methods L 8(2 7) orthogonal design and statistic analysis (SPSS 10.0) were used, the puerarin contents in the samples were determined by HPLC. Results Influence of Radix Scutellaria, Rhizoma Coptidis and Radix Glycyrrhizae Preparata on the puerarin content in GQD was insignificant. And interactions between two of three were insignificant too. In this experiment, puperarin in the sedimentation was found in the compatible decoction of Rhizoma Coptidis and Radix Puerariae. Conclusion The puerarin content is not markedly influenced by Radix Scutellaria, Rhizoma Coptidis and Radix Glycyrrhizae Preparata. Maybe, the change of pH is relevant to the sedimentation in the compatible decoction contained Rhizoma Coptidis. and Radix Puerariae
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[基金项目]
国家自然科学基金面上重点项目(39970886)