[关键词]
[摘要]
目的 研究人参总皂苷诱导白血病细胞凋亡及其机制 ,为进一步开发人参总皂苷提供实验依据。方法 采用细胞体外培养、图象分析技术、流式细胞术、形态学观察与免疫细胞化学等方法 ,研究人参总皂苷诱导 K5 6 2细胞凋亡。结果 人参总皂苷对 K5 6 2细胞有增殖抑制作用 ,并可诱导 K5 6 2细胞凋亡明显增加 ,同时 K5 6 2细胞癌基因产物 C- MYC,BCL- 2表达明显降低。结论 人参总皂苷能诱导 K5 6 2细胞凋亡 ,其机制可能与调控癌基因产物的表达有关。
[Key word]
[Abstract]
Object To clarify the mechanism for total saponins of Panax ginseng C. A. Mey. (TSPG)inducing K562 cells to apoptosis, and to provide the theoretical basis and the experimental evidence of TSPG 's clinical applicatio n. Methods By using in vitro cell culture, morphometry, flow cytometry , morphological investigation and immunocytochemistry , the effects of TSPG on apoptosis in K562 cells were studied. Results The results indicated that TSPG could inhibit the proliferation of K562 cells significantly , and induce K562 cells to apoptosis. It was also showed in the ex periments that after induced by TSPG,the ratio of positive K562 cells expressing C-MYC and BCL-2 is decreased. Conclusion The mechanism for TSPG to induce K562 cells to apoptosis may be related to the expression of oncogene in K562 cells.
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[基金项目]
国家自然科学基金项目(39670880);重庆市中医药科学基金项目