目的 建立改良的RP-HPlC测定人血清中阿魏浓度的分析方法。方法 RP-HPLC法,采用 Kromasil-C18柱为分析柱,甲醇-水-冰醋酸 (36.4∶63∶0 .6)为流动相,流速1.0mL / min,检测波长322 nm ,内标物为对羟基苯甲醛。用乙腈既去除人血清中的蛋白,又提取血清中的阿魏酸。结果 阿魏酸在 9.94～159.0 4ng/ mL 与峰面积呈良好线性关系 (r=0.9925) ,日内和日间RSD均<10%,平均回收率为 99.77%,最低检测浓度为5ng/mL。结论 该法简便、快速、灵敏、重现性好,低毒,适用于人血清中阿魏酸的定量分析。

Object To develop an improved RP-HPLC method for determining ferulicacid in the human serum. Methods The determination was carried on RP-HPLC, using Kromasi l-C18 column,methano l-water-acetic acid (36.4∶63∶0.6) as mobile phase with a flow rate of 1.0 mL /min and at th detection wavelength of 322nm, and p-hydroxy benzaldehyde was used as internal standard. The serum sample was deproteinized with acetoni trile to extract ferulic acid. Results The calibration curve showed good linearity over the range of 9.94-159.04 ng /mL (r= 0.9925) . RSD was less than 10% within day and day-to-day , the average recovery was 99. 77% and the minimal concent ration in serum was 5 ng /mL. Conclusion This method, which is simple, rapid, sensitive, reproducible and low toxic, is appropriate for the quantitative determination of ferulicacid in the human serum.

国家自然科学基金资助项目(No.30070912);高等学校骨干教师资助计划