[关键词]
[摘要]
目的 比较当归补血汤中的不同组分对正常及血虚小鼠模型的免疫调节作用。方法 采用MTT法测T、B淋巴细胞的增殖率;分光光度法检测脾细胞中抗体的生成;用乙酰苯肼和环磷酰胺制备小鼠血虚模型。结果 黄芪中的4个不同组分对正常小鼠均具有增强T、B淋巴细胞增殖率及促进抗体生成作用,而当归中的3个不同组分则作用各异,但是对血虚模型小鼠均表现出明显的免疫调节作用。体外实验发现:在2.5～250μg/mL浓度范围内,黄芪黄酮、黄芪皂苷以及黄芪和当归的挥发油部分均表现为良好的双向免疫调节作用,而黄芪和当归的多糖组分则为明显的浓度依赖性作用方式。结论 当归补血汤中的组分具有不同程度的免疫调节作用
[Key word]
[Abstract]
Object<\b> To study the immunomodulatory effect of seven fractions from DANGGUI BUXUE TANG in normal and blood deficiency mouse.Methods<\b> The proliferation of T, B lymphocyte was measured by MTT assay; the activity of splenocyte antibody was determined by spectroscopic assay; the blood deficiency model was prepared by injection of acetyl phenylhydrazine and cyclophosphamide.Results<\b> Four fractions from Astragalus membranaceus (Fisch.) Bunge all promoted proliferation of T and B lymphocyte and antibody production.Three fractions from Angelica sinensis (Oliv.) Diels had different effects at 200 mg/kg po in vivo, but they all showed significant immunomodulatory effects on blood deficiency mouse.In vitro, volatile oil, flavone and saponin from A.membranaceus and volatile oil, flavone from A.sinensis exhibited fine dual immunomodulatory effects, polysaccharide from both herbs showed well concentration dependent immunomodulatory effects at the doses of 2.5～250 μg/mL.Conclusion<\b> There are different immunomodulatory effects in the different fractions from DANGGUI BUXUE TANG.
[中图分类号]
[基金项目]
“九五”国家攀登计划预选项目:中药现代化关键问题的基础研究;编号98pd09
