目的 研究金银花药材道地性形成的基因基础。方法 用SDS法提取金银花Lonicera japonica不同居群、外类群细毡毛忍冬L．similis和山银花L．confusa的总DNA，进行5S－rRNA基因间区的PCR扩增和测序，并用软件Mega进行分析。结果 Lonicera L．属植物5S－rRNA基因间区约210bp，其中G+C含量较高，达70%左右，不同居群的L．japonica碱基序列有差别，通过测序可以进行鉴别。L．confusa与L．japonica间的遗传距离较大。结论 种间5S－rRNA基因间区的序列差异大于种内；道地药材之间的遗传距离较小；道地与非道地药材之间的遗传距离大于道地药材之间的遗传距离。

Object To study the formation of geoherbalism of Flos Lonicerae on the molecular level.Methods Genomic DNAs from different populations of Lonicera japonica Thunb. , outgroup Lonicera similis Hemsl. , and Lonicera conf usa DC. were extracted, the 5S-rRNA gene spacer region amplified, sequuenced, and analysed with Mega 1.02. Results The fragments of 5S-rRNA gene spacer region in Lonicera L. was about 210bp with G+ C content up to about 70% . The sequences were different in various populations, and can be identified by sequencing. The genetic distance between L.conf usa and L. japonica was larger. Conclusion The genuine distance between species was larger than that within species; the genetic distance among genuine crude drugs was smaller; the genetic distance among genuine and ungenuine crude drugs were bigger than that among genuine crude drugs.

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