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[摘要]
目的 建立复方蛇床子洗剂中蛇床子素定量分析的RP-HPLC方法。方法 选择醋酸甲地孕酮为定量内标物,采用Hypersil-ODS2(200mm×4.0mm,5μm)色谱柱,流动相为0.01mol/L磷酸二氢钠-乙腈(52∶48),流速1.2mL/min,检测波长320nm。结果 蛇床子素和内标在12min之内实现理想分离。在0.1238~2.970μg/mL范围内,蛇床子素与内标峰面积比与其相应浓度呈良好的线性关系(r=0.9996),方法平均回收率为(98.4±0.90)%,RSD为0.91%。结论 本法操作简便快速,定量准确可靠,可用于复方蛇床子洗剂的质量控制。
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[Abstract]
Object To estabish a method for the quantitative analysis of osthol in LOTIO FRUCTUS CNIDII COM POSITA by RP-HPLC. Methods Megestrol acetate was used as the internal standard. Results Chromatographic separation of osthol and megestrol acetate was accomplished within 12 min on Hypersil-ODS2 (200mm× 4.0 mm, 5μm) column and acetonitrile-0.01 mol/L sodium dihydrogen phosphate (48∶ 52) as mobile phase. The flow rate was 1.2 m L/min and the detection wavelength was 320nm. A good linearity was obtained in the range of 0.123 8~2.970μg /mL (r= 0.999 6) for osthol and the average method recovery was (98.4± 0.90)% with RSD= 0.91% . Conclusion The method was simple,rapid, accurate, reliable, and suitable for the quality control of LOT IO FRUCTUS CNIDII COM POSIT A.
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