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[摘要]
目的 为了选择贝母乙素含量较高的贝母原材料。方法 建立了 HPL C法分析贝母原材料中贝母乙素含量。贝母生药粉用氨水 - 95 %乙醇 (1∶ 5 )混合液提取 ,柱前用 2 ,4-二硝基苯肼 (DNPH)衍生 ,衍生化条件 5 0℃下反应 30 min。分析色谱柱 :Hypersil C1 8ODS2 ,流动相 :CH3CN- 10 0 mmol/ L CH3COONH4(4 1∶ 5 9,p H=5 .0 ) ,流速为1.2 m L/ min。U V375 nm检测。结果 4种市售贝母中东贝母的贝母乙素含量最高。结论 方法灵敏 ,重现性好 ,样品用量少 ,便于应用。
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[Abstract]
Object To develop a method for the quantitative determination of peiminine in bullus Fritillariae as a guide for the choice of a better product on the market. Methods An HPLC method was developed. The powdered drug was extracted with a mixed solution of ammonia-95% alcohol (1:5). The crude peiminine obtained was derivatized to its 2,4-dinitro hydrazone at pH 2.0 and 50?℃?for 30 min. The HPLC conditions were:Hypersil C18?column, with CH3CN:100 m Mol ammonium acetate (49:51) at pH 5.0 as the mobile phase and detected at the wave length of 375 nm. Results Among the 4 samples obtained from the market, F. thunbergii var. chekiangensis Hsiao et K. C. Hsia gave the highest peiminine content. Conclusion The method was highly sensitive with good reproducibility and need only a small amount of sample, suitable for practical use.
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