建立了一种可用于银杏离体细胞培养物中内酯A含量分析的RP-HPLC方法.样品经低浓度甲醇提取后,用中性或酸性氧化铝固相萃取柱纯化,用示差检测器检测,银杏内酯A的最低检测限为 0,012 μg,方法回收率为(96.0±2)%,RSD为 3.1%。

An analytical method was developed to confirm the production of ginkgolide A by Ginkgo biloba L. callus cultured in vitro. Biomass samples were selectively extracted with 10% methanol and the resulting extract was purified on aluminium oxide solid-phase extraction column. After concentration, the ginkgolide A was determined by HPLC on a C₁₈ column with refractive index detection. The recovery of the method was 96.0±2%. The detection limit was around 0.012 μg, which was low enough for the determination of ginkgolides produced by Ginkgo biloba L. callus cultured in vitro.

浙江省自然科学基金!（297066）