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[摘要]
对东北红豆杉、云南红豆杉、中国红豆杉和南方红豆衫的幼茎、针叶及其茎来源的愈伤组织的过氧化物酶(POD)、醋酶(EST)、多酚氧化酶(PPO)及超氧化物歧化酶(SOD)的同工酶,可溶性蛋白的含量及电泳谱带,多酚氧化酶和苯丙氨酸解氨酶(PAL)的活性作了比较研究。并探索了这几种同工酶的酶谱和两种酶活性与紫杉醇的生物合成的关系。
[Key word]
[Abstract]
The isoenzymes of peroxidase (POD), polyphenoloxidase (PPO), esterase (EST), and super oxide dismutase(SOD) in twigs,needles and calli of several Taxus species were studied using polyacrylamide gel electrophoresis (PAGE). The different enzymographic patterns of these isoenzymes on taxol biosynthesis were compared,and the activities of phenylalanine ammonialyase (PAL) and polyphenoloxidase tested to ex plore the relationships between taxol biosynthesis and these enzymes.
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