目的建立川芎油气相色谱指纹图谱的研究方法,以控制其质量。方法进样口温度为250℃,检测器温度为250℃,载气为氮气,体积流量为2mL/min,采用程序升温对10批川芎油的气相色谱进行分析,并采用GC-MS联用技术鉴定其指纹特征峰,并对指纹图谱进行相似度分析。结果60min内川芎油成分出峰完全。建立的川芎油指纹图谱共标定了13个特征峰,相似度高。结论本方法精密度、重现性、稳定性好,油中各成分分离较好,所建立的指纹图谱可作为川芎油的质量控制依据。

Objective To establish the method of the GC fingerprints for Ligusticum chuanxiong essential oil and its quality control.Methods The temperature at both feed inlet and detector was 250℃,the carrier gas was nitrogen,and the flow rate was 2 mL/min.GC of the essential oil in ten batches of L.chuanxiong was analyzed by adopting temprature programmer and the fingerprint peaks were identified by GC-MS.Results To set up the GC fingerprints for L.chuanxiong essential oil,13 peaks were marked out altogether and the GC fingerprints were analyzed by similarity analysis.Conclusion The method is of high degree of presion,reproducibility,and stability.The resolving of each component in the essential oil is good.The established fingerprints could be used as one of the quality control index for L.chuanxiong essential oil.

武汉市科技局专项资金资助项目(20056005130001)