目的研究泡沫分离法分离绞股蓝粗提液中皂苷的工艺。方法利用泡沫分离法对绞股蓝粗提液中的皂苷进行分离,以回收率和富集比为指标确定最佳分离工艺。结果最佳分离工艺为粗提液皂苷质量浓度为3.21μg/mL、气体流速30mL/min、塔装液量110mL、pH值9.0时,皂苷回收率为49.20%,富集比可达4.511。结论泡沫分离法是分离绞股蓝粗提液中皂苷的简单、有效方法。

Objective To study the method of extracting the gypenoside from crude Herba Gynostemmae Pentaphylli solution.Methods The gypenoside was extracted from crude Herba Gynostemmae Pentaphylli solution by foaming separation.The optimum conditions were obtained through calculating the recovery rate and enrichment ratio.Results Under the optimum conditions:the concentration of the gypenoside in crude Herba Gynostemmae Pentaphylli solution was 3.21 μg/mL,the gas flow rate was 30 mL/min,the volume of the solution was 110 mL,and pH value was 9.0 as well,the recovery rate of gypenoside was 49.20% and the enrichment ratio could reach 4.511.Conclusion Foam separation is a simple and effective method of extracting gypenoside from crude Herba Gynostemmae Pentaphylli solution.