目的 探究甘草次酸及其衍生物TY501对小鼠巨噬细胞RAW264.7增殖的影响。方法 以小鼠巨噬细胞RAW264.7为靶细胞，设置空白组，脂多糖（LPS）1 μg/mL处理组（模型组），LPS 1 μg/mL加80、40、20、10 μg/mL药物处理组（受试药物TY501，对照药物泼尼松龙、甘草次酸、吡罗昔康），每组设置4个复孔在给药刺激24 h后按照CCK-8试剂盒说明测定各药物对细胞增殖抑制率。结果 TY501半数抑制浓度（IC₅₀）为233 μg/mL，泼尼松龙IC₅₀为1 571 μg/mL，甘草次酸IC₅₀为31 μg/mL，吡罗昔康IC₅₀为14 187 μg/mL。结论 甘草次酸对RAW264.7巨噬细胞的增殖具有明显的抑制作用，甘草次酸衍生物TY501也可抑制小鼠巨噬细胞RAW264.7的增殖，在同等质量浓度条件下其对小鼠巨噬细胞增殖的抑制程度强于泼尼松龙，弱于甘草次酸，表明甘草次酸及其衍生物TY501对于小鼠巨噬细胞RAW264.7增殖的抑制作用可能是其发挥抗炎作用的机制之一。

Objective To investigate the effect of glycyrrhetinic acid and its derivative TY501 on the proliferation of murine macrophage RAW264.7. Methods Murine macrophage cells RAW264.7 were used as target cells, and the negative control group with only RAW264.7, model group with LPS 1 μg/mL treatment, and treatment groups with LPS 1 μg/mL plus 80, 40, 20, and 10 μg/mL of each drug (test drugs TY501, control drug prednisolone, glycyrrhetinic acid, and piroxicam) were set. Each group set four holes and after 24 h of administration, CCK-8 Kit was used for the determination of cell proliferation rates stimulated by different drugs. Results According to CCK-8 Kit determination results, half of the initial inhibition rate (IC₅₀) of the glycyrrhetinic acid was 31 μg/mL, TY501 IC₅₀ 233 μg/mL, Prednisolone 1 571 μg/mL, and Piroxicam 14 187 μg/mL. Conclusion The glycyrrhetinic acid has significantly inhibition on the proliferation of RAW264.7 as well as TY501, in a certain range of concentration, could inhibit the proliferation of murine macrophages RAW264.7. In the same degree of the concentration the inhibition of TY501 on cell proliferation is greater than those of Prednisolone and Piroxicam while less than that of glycyrrhetinic acid, which indicates the inhibition of glycyrrhetinic acid and its derivative TY501 on the proliferation of murine macrophage RAW264.7 may be one of the possible mechanisms of anti-inflammation.