Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures
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摘要:
目的：药用真菌瓦尼桑黄可在自然条件下合成具有药用价值的硬毛素类多酚。然而，由于其在自然条件下生长缓慢，野生瓦尼桑黄不能作为药物的主要来源。虽然在液体发酵培养中可以获得较多的菌丝体，但是硬毛素类多酚的积累量较少。 方法：在本研究中，采用多不饱和脂肪酸（PUFAs）、亚油酸（LA）、亚麻酸（ALA）和茉莉酸甲酯（MeJa）作为刺激因子来调节液体发酵培养条件下瓦尼桑黄生物量和硬毛素类多酚的积累。 结果：添加 LA 和 ALA 可促进菌丝体的积累，而添加 MeJa 则抑制瓦尼桑黄的生长，同时减少多酚总量。UPLC-Triple-TOF-MS分析表明，添加LA和ALA后，Hispidin、phellinstatin、pinnilidine及其衍生物的产量增加；添加MeJa后的第13天，hypholomine B及其异构体、3,14'-bihispidinyl和phelligridin E的产量增加。耐人寻味的是，添加 MeJa 的培养物中的总多酚具有很强的清除自由基的能力。化学结构分析表明，硬毛素类多酚具有较高的抗氧化活性。MeJa 诱导了更多的硬毛素类多酚的合成。 结论：添加 PUFAs 会影响瓦尼桑黄硬毛素类多酚的合成和组成成分。我们的研究结果为通过液体发酵培养瓦尼桑黄调控硬毛素类多酚的合成提供了参考。
Abstract:
Objective: The medicinal mushroom Sanghuangporus vaninii produces pharmaceutically valuable hispidin polyphenols in natural habitats. However, due to the slow growth in nature, S. vaninii grown in the field (sclerotia) is not reliable for pharmaceutical purposes. Although higher biomass of fungal mycelia can be obtained in submerged cultures, the accumulation of hispidin polyphenols is rare. Methods: In this study, the polyunsaturated fatty acids (PUFAs), linoleic acid (LA), linolenic acid (ALA), and methyl jasmonate (MeJa) were employed as the stimulant agents to coordinate the accumulation of biomass and hispidin polyphenols in its submerged cultures. Results: The addition of LA and ALA promoted the mycelial accumulation, while the addition of MeJa inhibited the growth of S. vaninii concomitant with reduced total polyphenols. UPLC-Triple-TOF-MS analysis revealed an increased production of hispidin, phellinstatin, pinnilidine, and its derivatives upon the addition of LA and ALA, and hypholomine B and its isomer, 3,14’-bihispidinyl, and phelligridin E upon the addition of MeJa on day 13. Intriguingly, total polyphenols from the MeJa-supplementing cultures harbored a high capacity in scavenging free radicals. Chemical structural analysis showed that hispidin polyphenols had higher antioxidant activity due to more hispidin moieties induced by MeJa. Conclusion: The supplement of PUFAs affects the synthesis and composition of hispidin polyphenols in S. vaninii. Our results provide a possibility to coordinate the production of hispidin polyphenols via submerged cultures of S. vaninii.
关键词:
硬毛素类多酚;亚油酸;亚麻酸;茉莉酸甲酯;瓦尼桑黄
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This study was supported by National Natural Science Foundation of China (No. 31170063 and 31470173) for Weifa Zheng.
Wenwen Yuan a, Weihua Yuan a, Rong Zhou a, Guoying Lv b, Mengni Sun a, Yanxia Zhao a,*, Weifa Zheng a,*. Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures[J]. Chinese Herbal Medicines (CHM),2023,15(4):594-602
