Directional screening and identification of potential cytotoxic components from Achnatherum inebrians by a combination of surface palsmon resonance and chromatography
Objective: To establish a method for directional screening of the cytotoxic components from the medicinal herb of Achnatherum inebrians by a combination of surface plasmon resonance (SPR) biosensor and chromatographic isolation technology. Methods: Under the guidance of bioactive assessment based on binding abilities between objects and the α-Mannosidase (α-Man) target, the active components from different solvents extracts, different polar extraction parts and fractions were screened orderly and directionally using SPR. Components with a high binding ability to a-Man can be precisely oriented in a narrower fractions range and are easy to isolate. Three human cancer cells were used to evaluate the cytotoxic activity of component with the highest affinity to α-Man. Results: Eight compounds were isolated and identificated from A. inebrians for the first time. Deoxyvasicinone possessed the highest affinity to α-Man among them. Moreover, deoxyvasicinone showed good effects on inhibited proliferation of human hepatoma cells HepG2 (IC50 = 5.7 lmol/L), human breast cancer cells MCF7 (IC50 = 7.21 lmol/L) and human lung cancer cells HCC827 (IC50 = 0.75 lmol/L), respectively. In particular, its inhibitory effect on HCC827 was stronger than the positive drug gefitinib (IC50 = 1.65 lmol/L). Conclusion: A comprehensive strategy of directional screening potential cytotoxic components from herb based on biomolecular interaction and chromatography was established. Deoxyvasicinone as an effective anti-cancer component was initially isolated from A. inebrians. It is expected that this screening strategy could provide new perspectives for rapid screening and identification of active components from natural plants with the complex matrix.
关键词:
醉马草;色谱分析法;细胞毒性成分;表面等离子体共振;α-甘露糖苷酶
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Project Supported:
The work was supported by the National Natural Science Foundation of China (No. 81573834), the Cultivation Project of Ideological and Political Demonstration Course for Graduate Courses of Minzu University of China (No. GRSKCSZ005), the Foundation of Key Laboratory of Ethnomedicine, Ministry of Education (No. KLEM-ZZ201808).
Wenbin Zhou a,, Man Wang a,, Aiqin Zhang b, Danrong Huang a, Hua Guo a, Gangyi Shen a,*. Directional screening and identification of potential cytotoxic components from Achnatherum inebrians by a combination of surface palsmon resonance and chromatography[J]. Chinese Herbal Medicines (CHM),2023,15(2):329-336
