Archive > Volume 15 Issue 2 > 2023,15(2):317-328. DOI:https://doi.org/10.1016/j.chmed.2022.06.009 Prev Next

基于UPLC-Q-Exactive-MS/MS 和 HPLC法对假东风草和东风草的化学成分分析和快速鉴别

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Chemical profiling and rapid discrimination of Blumea riparia and Blumea megacephala by UPLC-Q-Exactive-MS/MS and HPLC

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    摘要:
    目的：快速鉴定两种原植物形态和化学成分都非常相似的中药材假东风草和东风草，并研究二者的化学成分差异。 方法：利用UPLC-Q-Exactive-MS/MS分析和鉴定假东风草和东风草中的化学成分，进一步通过化学模式识别分析（CPR）比较和鉴别两种中药材，并确定其潜在的标志性成分。同时，建立了一种HPLC方法评价两种中药材的质量。 结果：从两种药材中共鉴定了93种成分，其中包括54种酚酸类成分，35种黄酮类成分，2种糖类成分，1种酚酸苷和1种其他成分，其中67种是在假东风草和东风草中首次鉴定。化学模式识别分析结果表明，根据LC-MS数据，可以明显区分假东风草和东风草。利用HPLC法得到的峰面积，计算异绿原酸A与隐绿原酸的峰面积比值可以区分假东风草和东风草，并对其药材质量进行控制。 结论：假东风草和东风草在化学成分方面存在显著差异。本实验为比较和评价假东风草和东风草的质量提供了一种快速、便捷的方法。

    Abstract:
    Objective: To rapidly identify the two morphologies and chemical properties of similar herbal medicines, Blumea riparia and B. megacephala as the basis for chemical constituent analysis. Methods: UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B. riparia and B. megacephala. Chemical pattern recognition (CPR) was further used to compare and distinguish the two herbs and to identify their potential characteristic markers. Then, an HPLC method was established for quality evaluation. Results: A total of 93 constituents are identified, including 54 phenolic acids, 35 flavonoids, two saccharides, one phenolic acid glycoside, and one other constituent, of which 67 were identified in B. riparia and B. megacephala for the first time. CPR indicates that B. riparia and B. megacephala samples can be distinguished from each other based on the LC–MS data. The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B. riparia and B. megacephala. Conclusion: This study demonstrates significant differences between B. riparia and B. megacephala in terms of chemical composition. The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B. riparia and B. megacephala.

    关键词:
    假东风草;东风草;化学模式识别;UPLC-Q-Exactive-MS/MS

    Keywords:


    Project Supported:
    We are grateful to Yanan Cao for her technical support of the UPLC-Q-Exactive-MS/MS. This study was supported by National Key Research and Development Program of China (No. 2018YFC1706101), The Sichuan Key Research and Development Program (No. 2021YFS0043), Leading Talent Support Program of National Civil Commission (2021), and Special Fund of Natural Science for the Central Universities (No. 2020NQN42).

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