Identification of Q-Markers from Hedan Tablet by employing "spider-web" mode and taking compounds hepatotoxicity into account
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摘要:
Abstract:
Objective: As a classic prescription in traditional Chinese medicine, Xuefu Zhuyu Decoction (XFZYD) has been widely used in the clinical treatment of cardiovascular and cerebrovascular diseases. In order to unveil the potentially effective compounds, a rapid ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was established to identify prototype components and their metabolites from XFZYD in rats' serum. Methods: The serum from rats after intragastric administration of XFZYD aqueous extract was analyzed by UPLC-Q-TOF/MS method. The prototype components and their metabolites were identified by comparison with the reference standards and tentatively characterized by comprehensively analyzing the retention time, MS data, characteristic MS fragmentation pattern and retrieving literatures. Results: A total of 175 components (24 prototype components and 151 metabolites) were identified and tentatively characterized. The metabolic pathways of prototype components in vivo were also summarized, including glucuronidation, hydrolyzation, sulfation, demethylation, and hydroxylation, and so on. Conclusion: In this study, a UPLC-Q-TOF/MS technique was developed to analyze prototype components and their metabolites from XFZYD in serum, which would provide the evidence for further studying the effective compounds of XFZYD.
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This work was supported by National Natural Science Foundation of China (No. 81873192 and 81202877 ) and Postgraduate Research and Innovation Project of Tianjin (No. 2019YJSS197).
Xiaoyu Zhang a, Zhenzuo Jiang a, Lei Zhang a, Cheng Xue a, Xiafei Feng b, Xin Chai a, Yuefei Wang a. Identification of Q-Markers from Hedan Tablet by employing "spider-web" mode and taking compounds hepatotoxicity into account[J]. Chinese Herbal Medicines (CHM),2022,14(4):612-621
