Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro
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Abstract:
Objective In this study, black tea and Citrus maxima (BT-CM), yellow tea and C. maxima (YT-CM), green tea and C. maxima (GT-CM) as subjects, the active ingredient content and antioxidant activity of three tea and C. maxima (T-CM) were analyzed. The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored. Methods National standard method and HPLC were used for active ingredient analysis. MTT, cell flow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation, apoptosis, and its underlying molecular mechanism. Results The content of tea polyphenols, free amino acids, ratio of polyphenols and amino acids, ester catechins, non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM. The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM. Three T-CMs had the effects of inhibiting proliferation, arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells, especially YT-CM and GT-CM. Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax, Bcl-2 and Caspase-3/9. YT-CM and GT-CM had better ability to change the signal pathway than BT-CM. Conclusion In short, T-CMs, which combined different degrees of fermentation tea with C. maxima, were rich in nutrients and biologically active substances. T-CMs, especially YT-CM and GT-CM, are healthy drinks that help to prevent and treat liver cancer.
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This work was supported by the National Natural Science Foundation of China (No. 81803236, 31800295, 81903319). Guangdong Science and Technology program (No. 2017A070702004, 2016B090918118, 2017A020224015, 2018KJYZ002), Natural Science Foundation of Guangdong Province (No. 2017A030310504), Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (No. R2018YJ-YB3002, R2019PY-JX004, R2016YJ-YB3003, R2018PY-QF005, R2018QD-101). Science and Technology Board of Yingde (No. JHXM2018029), Department of Science and Technology of Jiangmen (No. 2016350100170008351, 2018110100330005446), Department of Education of Guangdong Province (No. 2016KCXTD005, 2017KSYS010), and “14th Five-Year Plan” Team-building Projects of Guangdong Academy of Agricultural Sciences (No. 202126TD).
Shuai Wen, Ran An, Dong-li Li, Jun-xi Cao, Zhigang Li, Wenji Zhang, Ruohong Chen, Qiuhua Li, Xingfei Lai, Lingli Sun ,*, Shili Sun ,*. Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro[J]. Chinese Herbal Medicines (CHM),2022,14(3):
