A reliable and rapid pharmacokinetic study of pueraria isoflavones using pueraria reference extractive substance in beagle plasma: Application to study of Yufeng Ningxin Tablets
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摘要:
Abstract:
Objective: In order to evaluate the reliability and feasibility of pueraria reference extractive substance (RES) used in biological sample, the pharmacokinetics of 3 ? –hydroxy puerarin (3 ? -HP), puerarin, 3 ? – methoxy puerarin (3 ? -MP), and daidzein-8-C-apiosyl-(1-6)-glucoside (DAG) in beagle plasma following oral administration of Yufeng Ningxin Tablet were quantitated. Methods: A reliable and sensitive high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (HPLC-QQQ-MS/MS) method developed with chromatographic separation was oper- ated on a Merck C 18 column, and acetonitrile-5 mmol/L ammonium was used as mobile phase in gradient elution. The plasma samples were deproteinized by acetone, detected by triple quadrupole mass spec- trometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. The pharmacokinetic parameters were calculated by Winnonlin 4.1. Results: The calibration curves of the reference extractive substance and standard substance methods were linear over the ranges 0.0417–11.3309 μg/mL and 0.0394–10.0000 μg/mL. The intra-day and inter- day precision of the two methods at three concentrations were less than 13.63%, and the average recov- eries of 3 ? -HP, puerarin, 3 ? -MP, and DAG were more than 70.67%. The RSD of the mean plasma concentra- tions of the analytes calculated by the two methods was less than 5%, and cos (?) = =1.000. Among the analytes, puerarin showed the highest blood concentration [(940 ± 185) ng/mL] and the longest retention time [(5 ± 1) h] in the dog’s bodies. Conclusion: Pueraria reference extractive substance can be seen as an alternative to the standard sub- stance to overcome the scarcity of standard substance for the analysis of biological samples.
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We are grateful for financial support from the Major or Scien- tific and Technological Special Project for “Significant New Drug Creation” (No. 2014ZX0930437), the support of Conservation Prop- agation and Quality Standard of Characteristic Advantage of Chi- nese Herbal Medicine(No. 2015BAI05B03), Bijie City Achievement Transformation Project (Bi Ke Cheng Zi [2014] No. 8), Bijie City So- cial Development Project (Bi Kehe Zi [2015] No. 41).
Qin Zha a, b, Sha Chen a, Jun Zhang a, Xiang-yu Zhang b, Pei-jun Ruan b, Heba M. Amer c, An Liu a. A reliable and rapid pharmacokinetic study of pueraria isoflavones using pueraria reference extractive substance in beagle plasma: Application to study of Yufeng Ningxin Tablets[J]. Chinese Herbal Medicines (CHM),2020,12(1):88-94
